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	Provedor de dados Electron. J. Biotechnol. (1) Autor Brzozowski,Bartosz (1) Lewandowska,Malgorzata (1) Palavra-chave Central composite design (1) Plackett-Burman design (1) Response surface methodology (1) Tipo do documento Journal article (1) Ano 2014 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus Electron. J. Biotechnol. Brzozowski,Bartosz; Lewandowska,Malgorzata. Background Lactic acid bacteria are able to reduce the immunoreactivity of proteins of cereal grains during wheat dough fermentation or may be a source of proteolytic preparations added during bread making. The key enzyme in prolamin degradation is prolyl endopeptidase. This study was aimed at optimizing the composition of a culture medium and culture conditions that would enhance the synthesis of intracellular prolyl endopeptidase (PEP) by Lactobacillus acidophilus 5e2. Results The application of Plackett-Burman screening plans enabled demonstrating that the concentration of a nitrogen source in the culture and the initial pH value of the culture medium were significant for PEP synthesis. Further optimization conducted with the method of central composite... Tipo: Journal article Palavras-chave: Central composite design; Plackett-Burman design; Response surface methodology. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500003 Registros recuperados: 1 Primeira ... 1 ... Última

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