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Direct detection of toxic micro-algae Alexandrium minutum through high sensitive gravimetric sensors ArchiMer
Bucur, Bogdan; Lazerges, Mathieu; Perrot, Hubert; Antoine, Elisabeth; Hamelin, Muriel; Compere, Chantal.
In this work QCM technologies were used to set up a DNA based biosensor dedicated to the detection of toxic micro-algae in the environment. Up to now, appropriate detection were not achieved for different reasons as the possibility of regeneration, the capability of multidetection or the detection of very low DNA target concentration. For these reasons different configurations were examined based on in-situ and fast regeneration, supramolecular chemistry of DNA and high sensitive gravimetric devices. In a first step an OligoDesoxyRibonucleotide 5’ tailed with a disulfide group (SH-ODN) designed to be a specific molecular probe for the toxic micro-algae Alexandrium minutum was grafted on the quartz surface resonators. Kinetics of probe immobilization and of...
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/00089/19996/17659.pdf
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Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit ArchiMer
Garcia-martinez, Gonzalo; Alonso Bustabad, Enrique; Perrot, Hubert; Gabrielli, Claude; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodriguez-pardo, Loreto; Farina, Jose; Compere, Chantal; Arnau Vives, Antonio.
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
Tipo: Text Palavras-chave: DNA sequence detection; Quartz crystal microbalance; Electronic oscillator; Sensitivity; Frequency noise; Resolution.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00044/15487/12886.pdf
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