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	Provedor de dados BJMBR (1) Autor Bustamante,J.O. (1) Varanda,W.A. (1) Palavra-chave Cell nucleus (1) Gene activity (1) Gene expression (1) Ion channels (1) Macromolecular transport (1) Measurement (1) Nuclear envelope (1) Nuclear pores (1) Nucleocytoplasmic transport (1) Patch-clamp (1) Transcriptional control (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 1998 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Patch-clamp detection of macromolecular translocation along nuclear pores BJMBR Bustamante,J.O.; Varanda,W.A.. The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nuclear pores; Nuclear envelope; Cell nucleus; Nucleocytoplasmic transport; Macromolecular transport; Gene activity; Gene expression; Transcriptional control; Ion channels; Patch-clamp; Measurement. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003 Registros recuperados: 1 Primeira ... 1 ... Última

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