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In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) Rev. Bras. Bot.
CATAPAN,ELIZABETE; OTUKI,MICHEL FLEITH; VIANA,ANA MARIA.
An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 muM IBA. The best basal media for axillary shoot proliferation when 0.62 muM BA was supplemented were MS, MS/2 and AR (4-5 shoots per explant). Rooting was achieved with 100% of the microshoots on MS medium without growth regulators. Regenerated plants were successfully acclimatized and about 88% of plantlets survived under ex vitro conditions. Flowering was observed in 81% of the ex vitro grown plantlets after 12 weeks of acclimatization. High frequency callus initiation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shoot culture; Acclimatization; Ex-vitro flowering; Callus culture; Root culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003
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