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| CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; ALEXANDRE,RODRIGO S.. |
| Abstract This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya ‘Golden THB’. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya L; Malt extract; Myo-inositol; Polyethylene glycol. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703433 |
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| GOUVEA,DRIELLY S.; CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; SCHMILDT,OMAR; ARAUJO,CAROLINE P. DE; ALEXANDRE,RODRIGO S.. |
| Abstract: Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 μM) and 4-CPA (19, 22, 25, 28, and 31 μM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 μM) induced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abscisic acid; Auxins; Carica papaya L.; Gibberellic acid; Hermaphrodite explants; Polyethylene glycol. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500856 |
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