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Cloning of endothelin-1 (ET-1) from European sea bass (Dicentrarchus labrax) and its gene expression analysis in larvae with retinoic acid-induced malformations ArchiMer
Geay, Florian; Darias, Maria J.; Santigosa, E.; Desbruyeres, Elisabeth; Quazuguel, Patrick; Zambonino, Jose-luis; Cahu, Chantal; Mazurais, David.
It is known in vertebrates that endothelin-1 (ET-1) plays a key role ill morphogenesis whose expression level greatly influences the development of craniofacial malformations. In the Present study, the complete cDNA fragment encoding a precursor of endothelin-1, the preproendothelin-1 (PPET1), was cloned by RACE-PCR from European sea bass. The cDNA encoded a 199 amino acid polypeptide that was composed of the "mature" and "big" ET-1. Relative ET-1 expression levels were investigated in European sea bass larvae fed microparticulate diets containing the standard amount (0.08 g retinol/kg diet: group N) or all excess of retinoic acid which induces skeletal malformations (0.5 g retinol/kg diet: group RA). Real-time reverse transcriptor polymerase chain...
Tipo: Text Palavras-chave: European sea bass; Retinoic acid; Morphogenesis; Gene expression; Endothelin 1.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6172.pdf
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Domestication and nutrition in fish ArchiMer
Cahu, Chantal.
The aim of this paper is to evaluate to what extent, the study of feeding regimes of fish could be valuable to indicate the possibilities and the constraints of their domestication. Though the natural feeding regime of fish seems to be disadvantageous for their domestication, as protein requirement is high, different species could be considered as domesticated. These species are herbivorous, detritivorous, omnivorous as well as carnivorous. In the same way, there are large differences in gut anatomy, but domestication concerns species with or without stomach, and species with short or very long intestine. Nutritional requirements do not allow predicting species that could be domesticated. Some species seem to be able to use vegetable protein in...
Tipo: Text Palavras-chave: TROUT ONCORHYNCHUS MYKISS; TURBOT PSETTA MAXIMA; RAINBOW TROUT; TOTAL REPLACEMENT; FLESH QUALITY; LIVE FOOD; DIET; PROTEIN; GROWTH; LARVAE.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/11021/7571.pdf
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Dietary TAG source and level affect performance and lipase expression in larval sea bass (Dicentrarchus labrax) ArchiMer
Morais, Sophia; Cahu, Chantal; Zambonino-infante, Jose-luis; Robin, Jean; Ronnestad, I; Dinis, M; Conceicao, L.
The influence of dietary TAG source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mRNA level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. Fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. Coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain TAG. Growth was not related to lipase specific activity, suggesting a production in excess to dietary needs. Body lipid content was positively related to dietary lipid level and was affected by lipid quality. In addition, larval FA composition generally reflected that of the diet. The...
Tipo: Text Palavras-chave: Lipase activity; Dietary; Growth; Larvae; Sea bass; Fish culture.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-785.pdf
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Nutrition, digestion et développement des larves de crevettes et poissons marins ArchiMer
Cahu, Chantal.
This report is presented by Chantal Cahu for obtaining accreditation to supervise research. Since 1981, her research activity has addressed the study of reproducers' nutrition and the young stages of development of marine organisms belonging to two different branch lines, crustaceans and fish. This work, performed in a base-lined research body, IFREMER, is placed within a more general programme of aquaculture development. Indeed, whatever species are at issue, the development of aquaculture in France, as at the global level, is based on the production of juveniles in hatcheries. My work has therefore more precisely the goal of acquiring knowledge about the nutritional needs, mechanisms of digestion and the development of these animals in order to improve...
Tipo: Text Palavras-chave: Skeleton development; Vitamins; Nutriment; Fish larvae; Egg quality; Reproduction; Nutrition; Shrimp larvae; Peneids.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-2086.pdf
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An early-life hypoxia event has a long-term impact on protein digestion and growth in European sea bass juvenile. ArchiMer
Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy.
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined...
Tipo: Text Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf
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Do protein hydrolysates improve survival and growth of newly-hatched spotted, wolffish (Anarhichas minor), a non-metamorphic aquaculture fish species? ArchiMer
Savoie, A; Le Francois, Nathalie; Cahu, Chantal; Blier, P; Andreassen, I.
Despite larval robustness characterized by the absence of metamorphosis and readiness for exogenous feeding based on commercial feed, the spotted wolffish (Anarhichas minor) displays highly variable survival at first-feeding. In this study, we investigated the use of three dietary concentrations of protein hydrolysates (PH, pre-digested proteins) (0, 10 and 20%) when newly hatched juvenile wolffish were held at three different rearing temperatures namely, 5, 8 and 12 degrees C to determine whether digestion of protein was a limiting factor for fish growth and survival. Final weights for fish at 5, 8,and 12 degrees C at day 60 were respectively 0.35, 1.19 and 2.02 g. Mean specific growth rates were 1.97, 4.01 and 4.88%/day and survival rates were 49.8, 53.4...
Tipo: Text Palavras-chave: Survival; Growth; First feeding; Protein hydrolysates; Spotted wolffish; Anarhichas minor.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2141.pdf
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Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae ArchiMer
Kotzamanis, Y. P.; Gisbert, E.; Gatesoupe, Joel; Zambonino, Jose-luis; Cahu, Chantal.
Two Fish Protein Hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular weight of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae...
Tipo: Text Palavras-chave: Pseudoalteromonas Bacillus Vibrio; Marinomonas Bacterial challenge microbiota Fish hydrolysate Digestive enzymes Development Fish larvae.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2145.pdf
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Reduced lipid intake leads to changes in digestive enzymes in the intestine but has minor effect on key enzymes of hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss) ArchiMer
Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S.
For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced...
Tipo: Text Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf
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Evaluation of microdiets versus live feeds on growth, survival and fatty acid composition of larval haddock (Melanogrammus aeglefinus) ArchiMer
Blair, Tammy; Castell, Fisheries And Oceans Canada; Neil, Steven; D'Abramo, Louis; Cahu, Chantal; Harmon, Paul; Ogunmoye, Kehinde.
Until recently, it was considered impossible to feed newly hatched marine fish species with a compound diet. Substituting a compound diet for live prey was performed several weeks after hatching, depending on the species. Compound diets were well ingested at the early stage but larvae died with a gut full of food, suggesting that larvae were unable to digest the compound diet. The hypothesis was that younger larvae have insufficient digestive enzymes to thrive on compound diets, and that exogenous enzymes, provided from live prey, are necessary for early stages. The organogenesis of marine fish larvae is not completely achieved at hatching and histological studies have revealed that the anatomy of the digestive tract undergoes developmental changes over...
Tipo: Text Palavras-chave: Highly unsaturated fatty acids; Essential fatty acids; Survival; Growth; Artemia; Brachionus plicatilis; Microdiet; Haddock larvae.
Ano: 2003 URL: https://archimer.ifremer.fr/doc/2003/publication-445.pdf
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Biofloc technology applied to rear shrimp Litopenaeus Stylirostris broodstock: An integrated and development research project in New Caledonia and French Polynesia ArchiMer
Chim, Liet; Cardona, Emilie; Lorgeoux, Benedicte; Gueguen, Yannick; Saulnier, Denis; Goguenheim, Jean; Wantiez, Laurent; Cahu, Chantal.
Shrimp farming in New-Caledonia faces up to difficulties. A fluctuating quality of broodstock prevents sustainable production of larvae in quantity and quality. The traditional extensive rearing method of broodstock in outdoor earthen ponds used in New Caledonia raises several issues: poor water quality control and biosecurity. Therefore it is fundamental to modify the broodstock culture strategy in order to achieve an easier water quality management and maximal biosecurity. Biofloc technology (BFT) offers easier water quality management, higher natural productivity, higher level of biosecurity, and could be a good alternative. Since 2011 a R&D program started under the supervision of IFREMER in New Caledonia and French Polynesia in order to study and...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00250/36084/34635.pdf
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Effect of nature of dietary lipids on European sea bass morphogenesis: implication of retinoid receptors ArchiMer
Villeneuve, Laure; Gisbert, Enric; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal.
The effect of the nature and form of supply of dietary lipids on larval development was investigated in European sea bass larvae, by considering the expression of several genes involved in morphogenesis. Fish were fed from 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets incorporating different levels of EPA and DHA provided by phospholipid or neutral lipid. Phospholipid fraction containing 1 center dot 1 % (PL1 diet) to 2 center dot 3 % (PL3 diet) of EPA and DHA sustained good larval growth and survival, with low vertebral and cephalic deformities. Similar levels of EPA and DHA provided by the neutral lipid fraction were teratogenic and lethal. Nevertheless, dietary phospholipids containing high levels of DHA and EPA (PL5 diet)...
Tipo: Text Palavras-chave: Retinoic acid receptors; Skeletal malformations; Dicentrarchus labrax larvae; Neutral lipids; Phospholipids.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-880.pdf
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Probiotic treatment of live food organisms for Atlantic bluefin tuna larvae: microbiological & immunological criteria ArchiMer
Coves, Denis; De Vogue, Benoist; Desbruyeres, Elisabeth; Dhormes, Benoit; Fievet, Julie; Huelvan, Christine; Lallement, Stephane; Le Gall, Marie-madeleine; Ruelle, Francois; Vidal, Marie-odile; Castex, M.; Mazurais, David; Cahu, Chantal; Gatesoupe, François-joel.
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00051/16189/13702.pdf
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Dietary modulation of some digestive enzymes and Metabolic processes in developing marine fish: Applications to diet formulation ArchiMer
Zambonino, Jose-luis; Cahu, Chantal.
During these last 20 years, many studies have focussed on the development of the digestive tract in marine fish larvae. Most of the studies aimed at acquiring knowledge on the optimal form of dietary supply for different nutrients, in order to formulate a compound diet able to totally replace live preys in the fish larvae feeding sequence. Consequently, most of the studies aimed at describing the effect of dietary adaptation on digestive enzymes profile, the morphology of the main organs, while others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport...) were poorly investigated. This review reports the more recent data on dietary modulation of digestive enzymes with a particular emphasis, when possible, on the...
Tipo: Text Palavras-chave: Nuclear receptors; Digestive enzymes; Fatty acids; Vitamins; Dietary proteins and lipids; Fish larvae.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3045.pdf
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Gene expression patterns during the larval development of European sea bass (Dicentrarchus labrax) by microarray analysis ArchiMer
Darias, Maria J.; Zambonino, Jose-luis; Hugot, K; Cahu, Chantal; Mazurais, David.
During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were...
Tipo: Text Palavras-chave: Sea bass; Gene expression; Microarray; Development; Fish larvae.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4344.pdf
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FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax) ArchiMer
Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Tipo: Text
Ano: 2010
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Substitution of live food by formulated diets in marine fish larvae ArchiMer
Cahu, Chantal; Zambonino, Jose-luis.
Until recently, it was considered impossible to feed newly hatched marine fish species with a compound diet. Substituting a compound diet for live prey was performed several weeks after hatching, depending on the species. Compound diets were well ingested at the early stage but larvae died with a gut full of food, suggesting that larvae were unable to digest the compound diet. The hypothesis was that younger larvae have insufficient digestive enzymes to thrive on compound diets, and that exogenous enzymes, provided from live prey, are necessary for early stages.The organogenesis of marine fish larvae is not completely achieved at hatching and histological studies have revealed that the anatomy of the digestive tract undergoes developmental changes over...
Tipo: Text Palavras-chave: Protein; Phospholipid; Pancreatic enzymes; Microparticulate diet; Intestinal enzymes; Protein hydrolysate; Gut maturation; Fish larvae.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-447.pdf
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Biofloc contribution to antioxidant defence status, lipid nutrition and reproductive performance of broodstock of the shrimp Litopenaeus stylirostris: Consequences for the quality of eggs and larvae ArchiMer
Cardona, Emilie; Lorgeoux, Benedicte; Chim, Liet; Goguenheim, Jean; Le Delliou, Herve; Cahu, Chantal.
The aim of this study was to determine biofloc contributions to the antioxidant status and lipid nutrition of broodstock of Litopenaeus stylirostris in relationship with their reproductive performance and the health of larvae produced. Shrimp broodstock reared with Biofloc technology (BFT) compared to Clear water (CW) exhibited a higher health status with (i) a better final survival rate during the reproduction period (52.6% in CW against 79.8% in BFT); (ii) higher glutathione level (GSH) and total antioxidant status (TAS), reduced oxidized/reduced glutathione ratio and a higher spawning rate and frequency as well as higher gonado-somatic index and number of spawned eggs. Finally, larvae from broodstock from BFT exhibited higher survival rates at the Zoe 2...
Tipo: Text Palavras-chave: Biofloc technology; Shrimp broodstock culture; Litopenaeus stylirostris; Reproductive performance; Antioxidant defences; Fatty acids; Larval survival.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00275/38618/37148.pdf
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Ontogeny of the digestive tract of thick lipped grey mullet (Chelon labrosus) larvae reared in "mesocosms" ArchiMer
Zouiten, Dora; Ben Khemis, I; Besbes, R; Cahu, Chantal.
This work describes the ontogeny of the digestive tract in thick lipped grey mullet (Chelon labrosus) larvae reared until day 36 post-hatching with the semi-extensive technology in mesocosms. Diet was constituted by live preys, rotifers, Arternia and wild zooplankton, then compound diet was added from day 20 (p. h.). Linear growth, weight growth and digestive enzymes specific activities were studied during larval ontogeny. Pancreatic enzymes (trypsin and amylase) and intestinal enzymes (leucine-alanine peptidase "Leu-ala", aminopeptidase N "AN" and alkaline phosphatase "AP") were assayed in larvae sampled throughout the rearing trial to evaluate gastrointestinal maturation along the development. The trypsin specific activities were very high during the...
Tipo: Text Palavras-chave: Pancreatic enzymes; Intestinal enzymes; Fish larvae; Mesocosm; Chelan labrosus.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4585.pdf
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Ontogenèse des fonctions digestives et besoins nutritionnels chez les larves de poissons marins ArchiMer
Cahu, Chantal; Zambonino, Jose-luis.
The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first...
Tipo: Text Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf
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Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet ArchiMer
Geay, Florian; Culi, E. Santigosa I.; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y.; Corcos, L.; Bodin, Nathalie; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol...
Tipo: Text Palavras-chave: Sea bass (Dicentrarchus labrax); FADS2 expression and activity; HUFA biosynthesis.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11929/8635.pdf
Registros recuperados: 59
Primeira ... 123 ... Última
 

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