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Teruel,M.T.; Catalano,R.C.; Cabodevila,J.A.; Callejas,S.S.. |
Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos cultured for 24h in Ham-F10, CM5 or CM10 (49.66, 53.04, 60.00% respectively). However, CM5 significantly increased differentiation in embryos cultured for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo development; Mouse; Autoregulation; Trophic factors. |
Ano: 2005 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200005 |
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Uslenghi,G; Cabodevila,J; Callejas,S.S.. |
Se evaluó el efecto del cipionato de estradiol (CPE), de la Hormona Liberadora de Gonadotrofina (GnRH) y la combinación de ambos sobre la sincronización de ovulaciones y la tasa de preñez post inseminación artificial a tiempo fijo (IATF) en vacas de cría sin ternero al pie. En el Experimento 1, se utilizaron 40 vacas secas, las cuales se dividieron aleatoriamente para recibir cuatro inductores de la ovulación (n=10/grupo): 1 mg de CPE; 1 mg de benzoato de estradiol (BE), 10,5 μg de GnRH, o la combinación de CPE+GnRH. Se realizaron ecografías cada 12 horas para determinar el momento de ovulación. En el Experimento 2, las vacas recibieron los mismos tratamientos: Grupo CPE: n=71; Grupo BE: n=70; Grupo GnRH: n=67; Grupo CPE+GnRH: n=70. El Día 10 las... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Estrógeno; Buserelina; Inseminación artificial; Ovulación; Preñez. |
Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000200001 |
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