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| Lomonte,Bruno; Calvete,Juan J.. |
| Abstract This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Proteomics; Venomics; Antivenomics; Toxicovenomics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100206 |
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| Calvete,Juan J.; Petras,Daniel; Calderón-Celis,Francisco; Lomonte,Bruno; Encinar,Jorge Ruiz; Sanz-Medel,Alfredo. |
| Abstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venomics; Top-down proteomics; Top-down venomics; Protein species-resolved venomics; Absolute quantification; Inductively coupled plasma mass spectrometry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100205 |
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| Sanz,Libia; Pérez,Alicia; Quesada-Bernat,Sarai; Diniz-Sousa,Rafaela; Calderón,Leonardo A.; Soares,Andreimar M.; Calvete,Juan J.; Caldeira,Cleópatra A. S.. |
| ABSTRACT Background: The Brazil’s lancehead, Bothrops brazili, is a poorly studied pit viper distributed in lowlands of the equatorial rainforests of southern Colombia, northeastern Peru, eastern Ecuador, southern and southeastern Venezuela, Guyana, Suriname, French Guiana, Brazil, and northern Bolivia. Few studies have been reported on toxins isolated from venom of Ecuadorian and Brazilian B. brazili. The aim of the present study was to elucidate the qualitative and quantitative protein composition of B. brazili venom from Pará (Brazil), and to carry out a comparative antivenomics assessment of the immunoreactivity of the Brazilian antibothropic pentavalent antivenom [soro antibotrópico (SAB) in Portuguese] against the venoms of B. brazili and reference... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Bothrops brazili; Venomics; Third-generation antivenomics; Brazilian antibothropic polyvalent antivenom. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100308 |
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