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Variation and genetic structure of Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations based on ISSR pattern Genet. Mol. Biol.
Nascimento,Marcília A.; Batalha-Filho,Henrique; Waldschmidt,Ana M.; Tavares,Mara G.; Campos,Lucio A.O.; Salomão,Tânia M.F..
For a study of diversity and genetic structuring in Melipona quadrifasciata, 61 colonies were collected in eight locations in the state of Minas Gerais, Brazil. By means of PCR analysis, 119 ISSR bands were obtained, 80 (68%) being polymorphic. He and H B were 0.20 and 0.16, respectively. Two large groups were obtained by the UPGMA method, one formed by individuals from Januária, Urucuia, Rio Vermelho and Caeté and the other by individuals from São João Del Rei, Barbacena, Ressaquinha and Cristiano Otoni. The Φst and θB values were 0.65 and 0.58, respectively, thereby indicating high population structuring. UPGMA grouping did not reveal genetic structuring of M. quadrifasciata in function of the tergite stripe pattern. The significant correlation between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hymenoptera; Melipona; Genetic differentiation; ISSR markers; Population structure.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200031
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A molecular marker distinguishes the subspecies Melipona quadrifasciata quadrifasciata and Melipona quadrifasciata anthidioides (Hymenoptera: Apidae, Meliponinae) Genet. Mol. Biol.
Waldschmidt,Ana M.; Barros,Everaldo G. de; Campos,Lucio A.O..
The stingless bee species Melipona quadrifasciata includes two subspecies, Melipona quadrifasciata anthidioids and Melipona quadrifasciata quadrifasciata. The morphological difference between the two subspecies is the presence of three to five continuous yellow stripes on the terga on the 3rd to 6th segments in workers and males of M. q. quadrifasciata, and two to five interrupted bands in M. q. anthidioides. We identified a DNA marker which is present in M. q. quadrifasciata and absent in M. q. anthidioides. Only one among the M. q. quadrifasciata colonies did not present the marker. It was also absent in bees collected in northern Minas Gerais State (Brazil), despite their morphological resemblance to M. q. quadrifasciata. The marker can be used for...
Tipo: Info:eu-repo/semantics/other
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300019
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RAPD variation within and among species of Melittobia Westwood (Hymenoptera: Eulophidae) Neotropical Entomology
Sari,Eloisa H.R.; Tavares,Mara G.; Campos,Lucio A.O..
The genus Melittobia Westwood comprises several species of microparasitoids and only two of them are know to occur in Brazil up to now: M. australica Girault and M. hawaiiensis Perkins. Nevertheless, the differentiation between these two species using traditional taxonomy is very difficult. In the present study, we used random amplified polymorphic DNA chain reaction (RAPD-PCR) to test for its ability to discriminate between these two species and to examine the genetic variation among the studied populations of M. australica. Most of the generated fragments were species-specific, occurring in all individuals of one species and absent in the individuals of the other species demonstrating the appropriateness of such technique to distinguish between both of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melittobia australica; M. hawaiiensis; Molecular marker; Genetic variability; Microparasitoid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000200007
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Honey physicochemical properties of three species of the brazilian Melipona Anais da ABC (AABC)
Lage,Lorena G.A.; Coelho,Lívia L.; Resende,Helder C.; Tavares,Mara G.; Campos,Lucio A.O.; Fernandes-Salomão,Tânia M..
Physicochemical analyses were carried out to evaluate 27 samples of honeys from three species of the Brazilian genus Melipona (M. capixaba, M. rufiventris and M. mondury) from Espírito Santo and Minas Gerais States. The parameters water activity (Aw), percentage of soluble solids (Brix %), pH, acidity (meq/Kg) and moisture (%) were evaluated. The honey characteristics obtained from these samples were very similar to the ones from other Melipona species. However, regarding the honey from Apis (honey bee), only the pH values were similar. The low pH value and the high acidity detected in Melipona honey are potential factors for increasing the honey shelf life because they do not provide favorable conditions for the microbial development. On the other hand,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Honey; Melipona; Meliponini; Physicochemical data.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300003
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Manejo e manipulação artificial de colônias de Melipona quadrifasciata Lep. (Apidae: Meliponinae) Anais da SEB
Aidar,Davi S.; Campos,Lucio A.O..
Three methods of division of Melipona quadrifasciata Lep. colonies were evaluated: fisogastric queen naturally mated; orphans colonies, and queen mated in laboratory. Out of six colonies, five had grade 7 in 221 days, reaching 83,3% of success, in the 1st method. Out of four, 100% were formed using the 2nd method, in 221 days, and 71,4% out of seven colonies were formed with grade 7 in 195 days using the 3rd method. All colonies were treated with Syrup-A; the same management and same system of evaluation. Colonies obtained were described performance by the following mathematic models: Grade1 = 1.202 + 0.262 x reading; Grade2 = 1.474 + 0.250 x reading and Grade3 = 1.406 + 0.287 x reading. The 3rd method was the best considering time of the colonies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Hymenoptera; Beecolonies; Feeding; Fisogastric queens.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100021
Registros recuperados: 5
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