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Standardization of first and second-line antitubercular susceptibility testing using BacT Alert 3D system: a report from a tertiary care centre in India BJID
Nair,Deepthi; Capoor,Malini R.; Rawat,Deepti; Srivastava,Lakshmi; Aggarwal,Pushpa.
Resurgence of multidrug resistant tuberculosis has lead to demand for rapid susceptibility testing. Conventional methods take > 3 weeks and are tedious. Automated methods have superseded them for first line drug susceptibility testing. An attempt was made to standardize first and second line susceptibility testing using the BacT Alert 3D system (Biomerieux). And compare results with Lowenstein Jensen's (LJ) method. 121 isolates of Mycobacterium tuberculosis, 67 pulmonary and 54 extra pulmonary were subjected to sensitivity to first and second line drugs. Multidrug resistance was detected equally by both methods at 15.7%. 100% agreement was observed between the two methods for aminoglycosides, rifampicin, ethionamide and ciprofloxacin. 91.5% agreement...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antitubercular sensitivity; Second line; BacT Alert 3D system; LJ proportion.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600007
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Salmonella enterica serovar typhi: molecular analysis of strains with decreased susceptibility and resistant to ciprofloxacin in india from 2001-2003 BJID
Capoor,Malini R.; Nair,Deepthi; Aggarwal,Pushpa; Mathys,Vanessa; Dehem,Marie; Bifani,Pablo Juan.
Chromosomally-mediated reduced susceptibility to ciprofloxacin narrows the therapeutic options in enteric fever. We made a molecular comparison of clinical isolates of fluoroquinolone-resistant strains of Salmonella enterica serotype Typhi from January 2001 to May 2003; 178 isolates were subjected to antimicrobial susceptibility testing by the Kirby-Bauer method of disk diffusion, and agar dilution was used to determine the minimum inhibitory concentration (MIC) to ciprofloxacin. Nalidixic-acid resistant strains (NARST) were observed in 51% of the isolates, of which 98.9% had decreased susceptibility (MIC>0.125-1µg/mL) to ciprofloxacin. A single strain (4 µg/mL) was resistant to ciprofloxacin and double mutations were found in the gyrA gene (76...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella enterica; S.Typhi; Ciprofloxacin; Resistance.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400011
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Microflora of bile aspirates in patients with acute cholecystitis With or without cholelithiasis: a tropical experience BJID
Capoor,Malini R.; Nair,Deepthi; Rajni,; Khanna,Geetika; Krishna,S.V.; Chintamani,M.S.; Aggarwal,Pushpa.
The current study determined the spectrum of biliary microflora with special emphasis on enteric fever organisms in patients with acute cholangitis with and without cholelithiasis or other biliary diseases. The patients were divided into three groups: Group A consisted of patients with acute cholecystitis with cholelithiasis; Group B consisted of patients with acute cholecystitis with gastrointestinal ailments requiring biliary drainage and group C consisted of patients with gallbladder carcinoma. Gallbladder, bile and gallstones were subjected to complete microbiological and histopathological examination. Antimicrobial susceptibility of the isolates was performed as per CLSI guidelines. Bacteria were recovered from 17 samples (32%) in Group A, 17 (51.4%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bile; Acute cholecystitis; Salmonella enretica serovar Typhi.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300012
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Rapid diagnosis of community-acquired pneumonia using the Bac T/ alert 3D system BJID
Capoor,Malini R.; Nair,Deepthi; Aggarwal,Pushpa; Gupta,B..
We compared BacT/Alert 3D with conventional culture for the diagnosis of community-acquired pneumonia (CAP). Antimicrobial susceptibility testing of the isolates was performed with the disk diffusion method, and the minimum inhibitory concentration (MIC) was calculated. Automation was superior in terms of recovery and time to detect pathogens. The bacterial spectrum in CAP was Streptococcus pneumoniae (35.3%) Staphylococcus aureus (23.5%), Klebsiella pneumoniae (20.5%) and Haemophilus influenzae (8.8%). Three of the 12 S. pneumoniae isolates showed penicillin resistance on MIC and two showed erythromycin resistance. There were two H. influenzae strains resistant to penicillin; these were beta lactamase producers. One-fourth of the S. aureus were oxacillin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Community-acquired pneumonia; Streptococcus pneumoniae; Cefepime.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500010
Registros recuperados: 4
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