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Identification of duplicates in cassava germplasm banks based on single-nucleotide polymorphisms (SNPs) Scientia Agricola
Albuquerque,Hilçana Ylka Gonçalves de; Oliveira,Eder Jorge de; Brito,Ana Carla; Andrade,Luciano Rogério Braatz de; Carmo,Cátia Dias do; Morgante,Carolina Vianna; Vieira,Eduardo Alano; Moura,Elisa Ferreira; Faleiro,Fábio Gelape.
ABSTRACT: Genetic redundancy in cassava (Manihot esculenta Crantz) presents a challenge to efficient management of genetic resources. This study aimed to identify and define the genetic structure of duplicates in cassava germplasm from various Embrapa research units, using single-nucleotide polymorphism (SNP) markers. We evaluated 2,371 accessions with 20,712 SNPs. The identification of duplicates was performed based on multilocus genotypes (MLG), adopting a maximum genetic distance threshold of 0.05. The population structure was defined based on discriminant analysis of principal components (DAPC). A total of 1,757 unique and 614 duplicate accessions were identified. The redundancy of the collections ranged from 17 % (Belém, PA – Brazil) to 39 %...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta Crantz; Genotyping-by-sequencing; Multilocus; Genetic resources.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400328
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Molecular-assisted selection for resistance to cassava mosaic disease in Manihot esculenta Crantz Scientia Agricola
Carmo,Cátia Dias do; Silva,Maiane Suzarte da; Oliveira,Gilmara Alvarenga Fachardo; Oliveira,Eder Jorge de.
The geminivirus complex known as cassava mosaic disease (CMD) is one of the most devastating viruses for cassava (Manihot esculenta Crantz). The aim of this study was to use molecular-assisted selection (MAS) to identify CMD-resistant accessions and ascertain promising crosses with elite Brazilian varieties. One thousand two hundred twenty-four accessions were genotyped using five molecular markers (NS169, NS158, SSRY028, SSRY040 and RME1) that were associated with resistance to CMD, along with 402 SNPs (single-nucleotide polymorphism). The promising crosses were identified using a discriminant analysis of main component (DAPC), and the matrix of genomic relationship was estimated with SNP markers. The CMD1 gene, previously described in M. glaziovii, was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta; Breeding; Disease; Virus.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000600520
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