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Evaluation of lugol solution used for counting rumen ciliates Zoologia
D'Agosto,Marta; Carneiro,Maria Elisa.
The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rumen ciliate; Quantitative evaluation; Lugol solution; Technique.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
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Estudo comparativo do aspecto da hemolinfa de algumas espécies de carrapatos (Acari, Ixodidae): descrição da variação hemocitária de adultos de Amblyomma cajennense (Fabricius) Koch Zoologia
Carneiro,Maria Elisa; Daemon,Erik.
The hemocyte composition of adults of Amblyomma cajennense (Fabricius, 1787) Koch, 1844 adults consisted of three basic cellular types: prahemocyte I (PR I), plasmatocyte (PL) and spherulocyte (ES II). Among the ixodids observed (A. cajennense; A. dissimile Koch, 1844; Rhipicephalus sanguineus Latreille, 1806; Anocentor nitens (Neumann, 1897) Schulze, 1937; Boophilus microplus Canestrini, 1887 and Haemaphysalis sp.) only A. cajennense was different in the adult hemolymph colour. It changed from amber to deep blue as the ixodid age advanced. This fact could be associated with the presence of hemocyanin in the hemolymph and points out the need to develop further investigations about the Ixodidae hemolymph pratein composition as well as the function of all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ixodidae; Amblyomma; Ixodid; Hemolymph; Hemocyte.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752002000500011
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Caracterização dos tipos celulares presentes na hemolinfa de larvas e ninfas de Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) em diferentes estados nutricionais Zoologia
Carneiro,Maria Elisa; Daemon,Erik.
With the purpose of characterize morphologically the hemocytes of larvae and nymphs of Rhipicephalus sanguineus on different nutritional phases, it was obtained samples of haemolymph were obtained by sectioning the forelegs and collecting the drop there formed. After dried, the samples were fixed by methanol and stained by Giemsa. Microscopical observation resulted in the characterization of five basic cellular types: prohemocytes, plasmatocytes, granulocytes, spherulocytes and oenocytoids. Moreover, undefined cell types, whose morphological patterns didn't have correlation with the characteristics cited for hemocytes, were found in low frequency. The change in the relative composition of the haemolimph was characterized by decrease of the number of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ixodid; Rhipicephalus sanguineus; Hemocytes; Morphology.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751996000300010
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