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| Pacheco,Flávia Teresa Hansen; Silva-Stenico,Maria Estela; Etchegaray,Augusto; Gomes,José Elias; Carrilho,Emanuel; Tsai,Siu Mui. |
| Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus variegated chlorosis; Plant pathogen; Iron-transportation; Pyoverdine; Enterobactin; Polyketide synthase; Peptide synthetase. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026 |
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| Travensolo,Regiane F.; Carareto-Alves,Lucia M.; Costa,Maria V.C.G.; Lopes,Tiago J.S.; Carrilho,Emanuel; Lemos,Eliana G.M.. |
| Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylella fastidiosa; DNA microarray; Gene expression. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200022 |
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| Mazzu-Nascimento,Thiago; Melo,Débora Gusmão; Morbioli,Giorgio Gianini; Carrilho,Emanuel; Vianna,Fernanda Sales Luiz; Silva,André Anjos da; Schuler-Faccini,Lavinia. |
| Abstract Congenital anomalies are already the second cause of infant mortality in Brazil, as in many other middle-income countries in Latin America. Birth defects are a result of both genetic and environmental factors, but a multifactorial etiology has been more frequently observed. Here, we address the environmental causes of birth defects – or teratogens – as a public health issue and present their mechanisms of action, categories and their respective maternal-fetal deleterious effects. We also present a survey from 2008 to 2013 of Brazilian cases involving congenital anomalies (annual average of 20,205), fetal deaths (annual average of 1,530), infant hospitalizations (annual average of 82,452), number of deaths of hospitalized infants (annual average of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birth defects; Teratogens; Zika virus; Pregnancy; Public health. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300387 |
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| Lacava,Paulo Teixeira; Silva-Stenico,Maria Estela; Araújo,Welington Luiz; Simionato,Ana Valéria Colnaghi; Carrilho,Emanuel; Tsai,Siu Mui; Azevedo,João Lúcio. |
| The objective of this work was to study the production of siderophores by endophytic bacteria Methylobacterium spp., which occupy the same ecological niche as Xylella fastidiosa subsp. pauca (Xfp) in citrus plants. The siderophore production of Methylobacterium strains was tested according to chromeazurol agar assay test (CAS), Csáky test (hydroxamate-type) and Arnow test (catechol-type). In addition, the ability of Xfp to use siderophores, in vitro, produced by endophytic bacteria as source of iron, was evaluated. All 37 strains of Methylobacterium spp. tested were CAS-positive for siderophore production. Methylobacterium spp. produced hydroxamate-type, but not catechol-type siderophores. In vitro growth of Xfp was stimulated by the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus variegated chlorosis; Endophytes; Heterologous siderophores; Iron; Symbionts. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000400011 |
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| Mazzu-Nascimento,Thiago; Donofrio,Fabiana Cristina; Bianchi,Beatriz Cutilak; Travensolo,Regiane de Fátima; Souza Junior,João Batista; Moraes,Daniel Angeli; Morbioli,Giorgio Gianini; Segato,Thiago Pinotti; Varanda,Laudemir Carlos; Carrilho,Emanuel. |
| ABSTRACT Paper-based devices present low-cost and are versatile, making them very attractive for clinical analysis. To manufacture those devices wax patterns are printed on paper surface and upon heating the wax permeates through the entire thickness of the paper, creating hydrophobic barriers that delimit test areas. Antibodies produced in rabbits against canine distemper virus (CDV) were physically adsorbed on the surface of gold nanoparticles (AuNPs) and incubated with CDV viral antigens, forming the immunocomplex. Anti-CDV antibodies were immobilized into the microchannels by physical adsorption, forming the test region. The test solution containing conjugated AuNPs was applied at the bottom of the microchannel and it was eluted with a phosphate buffer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paper-based devices; Wax printing; Point-of-care testing; Gold nanoparticles; Canine distemper virus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100318 |
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| Silva,Tatiane M.; Stets,Maria I.; Mazzetto,André M.; Andrade,Fabiana D.; Pileggi,Sônia A. V.; Fávero,Paulo R.; Cantú,Marcelo D.; Carrilho,Emanuel; Carneiro,Paulo I.B.; Pileggi,Marcos. |
| The aim of this work was to isolate microorganisms from Brazilian soil contaminated with 2,4-D herbicide, and analyze the efficiency for 2,4D degradation, using high-performance liquid chromatography (HPLC). Serratia marcescens and Penicillium sp had never been reported as able to degrade 2,4-D. The isolated strains represent a great potential for bioremediation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioremediation Biodegradation 2; 4-dichlorophenoxyacetic acid. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300026 |
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