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Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil BJID
Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Chamon,Raiane C.; Santos,Kátia R.N. dos; Marques,Lucas M..
ABSTRACT This study characterized 30 MRSA isolates from intensive care unit (ICU) environment and equipment surfaces and healthy children. The SCCmec types I, IVa and V were detected in HA-MRSA isolates while CA-MRSA showed the SCCmec type IVa and V. Most isolates were classified as agr group II. All isolates presented the sei gene, and only HA-MRSA were positive for etb e tst genes. Three genotypes were related to Pediatric (ST5/SCCmecIV) and Berlin (ST45/SCCmecIV) clones. The present study showed molecular similarity between CA- and HA-MRSA isolates in hospital and community settings in a Brazilian region.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Methicillin-resistant Staphylococcus aureus; CA-MRSA; HA-MRSA; Molecular typing.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200134
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Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil BJID
Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Oliveira,Arianne C. de; Nascimento,Flávia S.; Campos,Guilherme B.; Oliveira,Márcio V.; Timenetsky,Jorge; Marques,Lucas M..
Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CA-MRSA; Antibiotic resistance; SCCmec; MLST.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400464
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