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	Provedor de dados Genet. Mol. Biol. (3) Autor Carvalho-Salles,Andréa Borduchi (3) Tajara,Eloiza Helena (3) Barril,Nilce (2) Fett-Conte,Agnes Cristina (1) Richieri-Costa,Antonio (1) Sandrin-Garcia,Paula (1) Mais... Palavra-chave 22q11.2 deletion (1) Velocardiofacial syndrome (1) Tipo do documento Info:eu-repo/semantics/article (3) Ano 2007 (1) 1999 (1) 1998 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A simple procedure for rehybridization of nuclei analyzed previously by fish Genet. Mol. Biol. Carvalho-Salles,Andréa Borduchi; Barril,Nilce; Tajara,Eloiza Helena. We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available. Tipo: Info:eu-repo/semantics/article Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006 Fluorescence in situ hybridization (FISH) screening for the 22q11.2 deletion in patients with clinical features of velocardiofacial syndrome but without cardiac anomalies Genet. Mol. Biol. Sandrin-Garcia,Paula; Richieri-Costa,Antonio; Tajara,Eloiza Helena; Carvalho-Salles,Andréa Borduchi; Fett-Conte,Agnes Cristina. The velocardiofacial syndrome (VCFS), a condition associated with 22q11.2 deletions, is characterized by a typical facies, palatal anomalies, learning disabilities, behavioral disturbances and cardiac defects. We investigated the frequency of these chromosomal deletions in 16 individuals with VCFS features who presented no cardiac anomalies, one of the main characteristics of VCFS. Fluorescent in situ hybridization (FISH) with the N25 (D22S75; 22q11.2) probe revealed deletions in ten individuals (62%). Therefore, even in the absence of cardiac anomalies testing for the 22q11.2 microdeletions in individuals showing other clinical features of this syndrome is recommended. Tipo: Info:eu-repo/semantics/article Palavras-chave: Velocardiofacial syndrome; 22q11.2 deletion. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100006 A rapid technique for analysis of formalin-fixed, paraffin-embedded tissues by fluorescent in situ hybridization with alpha-satellite probes Genet. Mol. Biol. Barril,Nilce; Carvalho-Salles,Andréa Borduchi; Tajara,Eloiza Helena. We describe a rapid procedure for preparing archival tissues for interphase FISH analysis. The present protocol differs from others previously described because it allows the obtention of nuclei in satisfactory number and quality without using special equipments, adhesive-treated slides or solutions for chromatin decondensation. The method is of low cost and useful for retrospective analyses of formalin-fixed, paraffin-embedded samples. Tipo: Info:eu-repo/semantics/article Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400006 Registros recuperados: 3 Primeira ... 1 ... Última

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