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| Castejón,Orlando J.. |
| The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001 |
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| Castejón,Orlando J.. |
| The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fibers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calbindin; Synapsin-PSD95; GluR1; CamKII; N-cadherin. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200003 |
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| Castejón,Orlando J.; Dailey,Michael E.. |
| The localization of GluR1 subunits of ionotropic glutamate receptors in the glial cells and inhibitory neurons of cerebellar cortex and their association with the climbing and parallel fibers, and basket cell axons were studied. Samples of P14 and P21 rat cerebellar cortex were exposed to a specific antibody against GluR1 subunit(s) of AMPA receptors and were examined with confocal laser scanning microscopy. GluR1 strong immunoreactivity was confined to Purkinje cell and the molecular layer. Weak GluR1 immunoreactivity was observed surrounding some Golgi cells in the granule cell layer. Intense GluR1 immunoreactivity was localized around Purkinje, basket, and stellate cells. Purkinje cells expressed strong GluR1 immunoreactivity surrounding the cell body,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutamate receptors; Glia; Neurons; Confocal microscopy. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200001 |
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