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| Ferraz,Rafaela Siqueira; Mendonça,Elisângela Afonso Moura; Silva,Jéssica Priscila Avelino; Cavalcanti,Isabella Macário Ferro; Lira-Nogueira,Mariane Cajubá Britto; Galdino,Suely Lins; Pitta,Ivan Rocha; Lima,Maria do Carmo Alves; Santos-Magalhães,Nereide Stela. |
| The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 µg.mL−1 (Absorbance=0.18068 x [LPSF/AC04 µg.mL-1] + 0.00348), (r2=0.9995). The limits of detection and quantification were 0.047µg.mL−1 and 0.143µg.mL−1, respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: UV Spectrophotometry/quantitative analysis/method validation; Acridine derivatives/determination; Inclusion complex; Liposomes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100183 |
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| Cavalcanti,Isabella Macário Ferro; Menezes,Talita Gomes Calaça; Campos,Luís André de Almeida; Ferraz,Milena Sales; Maciel,Maria Amélia Vieira; Caetano,Maria Nelly Psiotano; Santos-Magalhães,Nereide Stela. |
| ABSTRACT The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-lapachone; Usnic acid; Liposomes; Methicillin-resistant Staphylococcus aureus (MRSA); Synergism.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200627 |
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