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Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge BABT
Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003
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Influence of high osmotic pressure on sorbitol production by Zymomonas mobilis BABT
Vignoli,Josiane Alessandra; Cazetta,Marcia Luciana; Silva,Rui Sérgio Santos; Celligoi,Maria Antonia Pedrine Colabone.
The objective of the present work was to study the variation on the sorbitol production in relation to the concentration of sugars, (metabolizable or not) and the cultivation time. A full factorial design was used considering the factors such as sucrose and maltose concentration and cultivation time. The addition of sugars caused increases on the sorbitol production up to the concentration of 300g/L however, decreases on the sorbitol production were observed when the concentration reached values above this. Increasing the time of fermentation was statistically significant to sorbitol production, however, little increase the production was noticed after 36h.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymomonas mobilis; Sorbitol; High osmotic stress; Maltose.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500022
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Levan from Bacillus subtilis Natto: its effects in normal and in streptozotocin-diabetic rats BJM
Melo,Fernando Cesar Bazani Cabral de; Zaia,Cássia Thaïs Bussamra Viera; Celligoi,Maria Antonia Pedrine Colabone.
Levan is an exopolysaccharide of fructose primarily linked by β-(2→6) glycosidic bonds with some β-(2→1) branched chains. Due to its chemical properties, levan has possible applications in both the food and pharmaceutical industries. Bacillus subtilis is a promising industrial levan producer, as it ferments sucrose and has a high levan-formation capacity. A new strain of B. subtilis was recently isolated from Japanese food natto, and it has produced levan in large quantities. For future pharmaceutical applications, this study aimed to investigate the effects of levan produced by B. subtilis Natto, mainly as potential hypoglycemic agent, (previously optimized with a molecular weight equal to 72.37 and 4,146 kDa) in Wistar male rats with diabetes induced by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Levan; Bacillus subtilis Natto; Hypoglycemia.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400046
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Synthesis of sorbitol by Zymomonas mobilis under high osmotic pressure BJM
Barros,Márcio de; Celligoi,Maria Antonia Pedrine Colabone.
The bacterium Zymomonas mobilis presents potential for sorbitol production when grown in culture medium with high sugar concentration. Sorbitol is produced and accumulated in the periplasma of the bacterium to protect the cells from the harmful effects of high osmotic pressure that results from the action of invertase on sucrose. The conversion of sucrose into glucose and fructose increases the osmolarity of the medium. However, an excessive increase in the osmotic pressure may decrease the sorbitol production. In this work Saccharomyces cerevisiae invertase was added two media containing sucrose 200 and 300 g.L-1. Sorbitol production in sucrose at 200 g.L-1 was 42.35 and 38.42 g.L-1, with and without the invertase treatment, respectively. In the culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymomonas mobilis; Invertase; Osmotic pressure; Sorbitol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300023
Registros recuperados: 4
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