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| Matallo,M.B; Almeida,S.D.B; Cerdeira,A.L; Franco,D.A; Blanco,F.M.G; Menezes,P.T.C; Luchini,L.C; Moura,M.A.M; Duke,S.O. |
| A better method for determination of shikimate in plant tissues is needed to monitor exposure of plants to the herbicide glyphosate [N-(phosphonomethyl)glycine] and to screen the plant kingdom for high levels of this valuable phytochemical precursor to the pharmaceutical oseltamivir. A simple, rapid, and efficient method using microwave-assisted extraction (MWAE) with water as the extraction solvent was developed for the determination of shikimic acid in plant tissues. High performance liquid chromatography was used for the separation of shikimic acid, and chromatographic data were acquired using photodiode array detection. This MWAE technique was successful in recovering shikimic acid from a series of fortified plant tissues at more than 90% efficiency... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyphosate; Green chemistry; Herbicide; HPLC. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582009000500012 |
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