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	Provedor de dados BJM (1) Ciência Rural (1) Autor Cerqueira,Gustavo Maia (2) Simionatto,Simone (2) Conceição,Fabrício Rochedo (1) Dellagostin,Odir Antonio (1) Dellagostin,Odir Antônio (1) Galli,Vanessa (1) Lagranha,Valeska Lizzi (1) Luerce,Tessália Diniz (1) Michelon,André (1) Michelon,Marcelo (1) Vaz,Eliana Knackfus (1) Mais... Palavra-chave DNA vaccine (1) FaeC (1) Kozak sequence (1) PCR-REA (1) PSE pork (1) Plucked hair (1) Swine colibacilosis (1) Swine stress gene (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effect of the Kozak sequence on seroconversion of mice immunized with a DNA vaccine against swine colibacilosis BJM Michelon,André; Michelon,Marcelo; Simionatto,Simone; Lagranha,Valeska Lizzi; Conceição,Fabrício Rochedo; Vaz,Eliana Knackfus; Cerqueira,Gustavo Maia; Dellagostin,Odir Antonio. The neonatal diarrhea in swine caused by enterotoxigenic Escherichia coli (ETEC) is responsible for high mortality and low growth rate in pigs and it is mainly dependent on the capacity of E. coli to attach to the surface of the small intestine, a property mediated by fimbria. In this study the faeC gene, which codes for the minor fimbrial subunit of E. coli K88ab, was cloned in the eukaryotic expression vector pcDNA3, associated or not to the Kozak sequence. Plasmid DNA of the two versions of the vaccine candidate was inoculated in mice by the intramuscular route, in two doses, at 0 and 21 days. The animals that received the DNA vaccine containing faeC associated to the Kozak sequence presented seroconversion significantly higher (P<0.05) than the one... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine colibacilosis; DNA vaccine; FaeC; Kozak sequence. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500029 An improved method for characterization of the mutation associated to porcine stress syndrome by PCR amplification followed by restriction analysis Ciência Rural Luerce,Tessália Diniz; Galli,Vanessa; Cerqueira,Gustavo Maia; Simionatto,Simone; Dellagostin,Odir Antônio. A mutation in the gene coding for the ryanodine receptor 1 (RYR1), also known as halothane (hal) gene or swine stress gene, is associated to the porcine stress syndrome (PSS). Detection of the mutation is normally accomplished by PCR amplification of an 81bp fragment of the hal gene, followed by digestion with the HhaI restriction endonuclease. Wild-type allele (N) is cut in two fragments, whereas the mutant allele (n) is not digested by the restriction enzyme. Electrophoresis of the digested DNA on agarose gel and ethidium bromide staining allows the reading of the result. The correct interpretation is difficult due to the small size of the DNA fragments. In this study we designed a new set of primers for amplification of a 144bp fragment that facilitates... Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR-REA; Plucked hair; Swine stress gene; PSE pork. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000500044 Registros recuperados: 2 Primeira ... 1 ... Última

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