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NanoSIMS single cell analyses reveal the contrasting nitrogen sources for small phytoplankton ArchiMer
Berthelot, Hugo; Duhamel, Solange; L’helguen, Stéphane; Maguer, Jean-francois; Wang, Seaver; Cetinić, Ivona; Cassar, Nicolas.
Nitrogen (N) is a limiting nutrient in vast regions of the world’s oceans, yet the sources of N available to various phytoplankton groups remain poorly understood. In this study, we investigated inorganic carbon (C) fixation rates and nitrate (NO3−), ammonium (NH4+) and urea uptake rates at the single cell level in photosynthetic pico-eukaryotes (PPE) and the cyanobacteria Prochlorococcus and Synechococcus. To that end, we used dual 15N and 13C-labeled incubation assays coupled to flow cytometry cell sorting and nanoSIMS analysis on samples collected in the North Pacific Subtropical Gyre (NPSG) and in the California Current System (CCS). Based on these analyses, we found that photosynthetic growth rates (based on C fixation) of PPE were higher in the CCS...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00462/57383/64185.pdf
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