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| Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.. |
| In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012 |
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| Colariccio,Addolorata; Chaves,Alexandre L.R.; Eiras,Marcelo; Chagas,César M.; Roggero,Piero. |
| During surveys undertaken from 1998 to 2003 in the major vegetable growing areas of the city of São Paulo green belt, lettuce (Lactuca sativa) and endive (Cichorium endivia) plants were observed, which showed chlorotic thickening of foliar veins, defective growth and, in some cases, failure to form complete heads. Biological and serological [DAS-Enzyme linked immunosorbent assay (Elisa)] tests together with electron microscope observations, revealed the presence of Lettuce big-vein virus and Mirafiori lettuce virus, in these plants both responsible for the lettuce big-vein syndrome. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Lettuce big-vein associated virus; Mirafiori lettuce virus; Lettuce; Virus transmitted by fungi. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400014 |
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| Gonçalves,Marcos C.; Santos,Amaury S.; Maia,Ivan G.; Chagas,César M.; Harakava,Ricardo. |
| Os vírus pertencentes ao subgrupo do Sugarcane mosaic virus (SCMV, gênero Potyvirus, família Potyviridae) infectam e causam mosaico em diferentes espécies botânicas da subfamília Panicoideae (família Poaceae), porém apenas o SCMV e o Sorghum mosaic virus (SrMV) infectam naturalmente cana-de-açúcar. No Brasil, a espécie SCMV parece ser o único agente causal da doença. Embora a maioria das variedades comerciais de cana-de-açúcar seja considerada resistente ou tolerante ao SCMV, relatos de incidência de mosaico em tais variedades têm ocorrido no campo. Amostras com sintomas, de diversos clones e variedades, foram coletadas em campos experimentais e comerciais de cana-de-açúcar. Dentre as variedades, a RB72-454, uma das mais plantadas no país e considerada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potyviridae; Mosaico da cana; Sugarcane mosaic virus subgroup. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000100004 |
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