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Screening and kinetics of glutaminase and glutamate decarboxylase producing lactic acid bacteria from fermented Thai foods Ciênc. Tecnol. Aliment.
Woraharn,Sasimar; Lailerd,Narissara; Sivamaruthi,Bhagavathi Sundaram; Wangcharoen,Wiwat; Sirisattha,Sophon; Chaiyasut,Chaiyavat.
L-glutaminase and glutamic acid decarboxylase (GAD) catalyzes the hydrolysis of L-glutamine and glutamate, respectively. L-glutaminase widely used in cancer therapy along with a combination of other enzymes and most importantly these enzymes were used in food industries, as a major catalyst of bioconversion. The current investigation was aimed to screen and select L-glutaminase, and GAD producing lactic acid bacteria (LAB). A total of 338 LAB were isolated from fermented meat, fermented fish, fermented soya bean, fermented vegetables and fruits. Among 338 isolates, 22 and 237 LAB has been found to be positive for L-glutaminase and GAD, respectively. We found that 30 days of incubation at 35 ºC and pH 6.0 was the optimum condition for glutaminase activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L-glutaminase; Glutamic acid decarboxylase; Lactic acid bacteria and 16S rRNA sequencing.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000400022
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The influence of extraction methods on composition and antioxidant properties of rice bran oil Ciênc. Tecnol. Aliment.
Pengkumsri,Noppawat; Chaiyasut,Chaiyavat; Sivamaruthi,Bhagavathi Sundaram; Saenjum,Chalermpong; Sirilun,Sasithorn; Peerajan,Sartjin; Suwannalert,Prasit; Sirisattha,Sophon; Chaiyasut,Khontaros; Kesika,Periyanaina.
AbstractThe current study was employed to assess the influence of the different extraction methods on total tocols, γ-oryzanol content, and antioxidant properties of Chiang Mai Black rice, Mali Red rice, and Suphanburi-1 Brown rice bran oil. Rice bran oil (RBO) was extracted by Hexane, Hot pressed, Cold pressed, and Supercritical Fluid Extraction (SFe) methods. High yield of RBO was extracted by hexane and SFe methods. Total and subgroups of tocols, and γ-oryzanol content were determined by HPLC. The hexane extracted sample accounts for high content of γ-oryzanol and tocols. Besides, all of RBO extracts contain a significantly high amount of γ-tocotrienol. In vitro antioxidant assay results indicated that superior quality of oil was recovered by hexane...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rice bran oil; Tocols; Γ-oryzanol; Antioxidants.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000300493
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Physicochemical and antioxidative properties of black, brown and red rice varieties of northern Thailand Ciênc. Tecnol. Aliment.
Pengkumsri,Noppawat; Chaiyasut,Chaiyavat; Saenjum,Chalermpong; Sirilun,Sasithorn; Peerajan,Sartjin; Suwannalert,Prasit; Sirisattha,Sophon; Sivamaruthi,Bhagavathi Sundaram.
Rice, the seed of Oryza species, is the major cereal crop in most of the developing countries. Nearly 95% of global rice production is done in Asian countries, and about half of the world’s population consumes it. Some speciality rices are not commonly consumed. Colored rice is one of such variety. In these varieties, high amounts of anthocyanin pigment are deposited in the rice coat to form its black (also known as purple), brown and red colors. Minimum studies are there to explain the properties of these rice varieties of Thailand. Thus, the current study was aimed to assess the physicochemical and antioxidative properties of three rice varieties (Chiang Mai Black rice, Mali Red rice and Suphanburi-1 Brown rice) of different cultivars of northern...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-oxidant; Phytochemicals; Colored rice variety; Polar and non-polar extraction.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000200331
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Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Electron. J. Biotechnol.
Kantachote,Duangporn; Prachyakij,Pakorn; Charernjiratrakul,Wilawan; Ongsakul,Metta; Duangjitcharoen,Yodsawee; Chaiyasut,Chaiyavat; Nitoda,Teruhiko; Kanzaki,Hiroshi.
Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B12 and the presence and absence of oxygen. Its inhibitory effect against food borne pathogenic bacteria and spoilage...
Tipo: Journal article Palavras-chave: Fermented plant beverage; Lactobacillus plantarum; Phenyllactic acid; Probiotics; Rhodotorula mucilaginosa; Weight control.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500002
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Enhancement of γ-aminobutyric acid in a fermented red seaweed beverage by starter culture Lactobacillus plantarum DW12 Electron. J. Biotechnol.
Ratanaburee,Anussara; Kantachote,Duangporn; Charernjiratrakul,Wilawan; Penjamras,Pimpimol; Chaiyasut,Chaiyavat.
Lactobacillus plantarum DW12, a gamma-aminobutyric acid (GABA) producing strain, was used as a starter culture to produce a functional fermented red seaweed beverage (FSB). Optimal conditions for producing FSB were established using Central Composite Design by varying the amounts of monosodium glutamate (MSG), sucrose and the initial pH in MRS medium. After a verification test, 1% MSG, 6% sucrose and an initial pH of 6 were selected. Four treatments were tested: traditional formula (A), red seaweed-cane sugar-potable water = 3:1:10, w/w/v, initial pH 6; the traditional formula with a 5% starter culture consisting of 4.1 x 10(9) CFU of DW 12/ml (B); formula A modified by changing the amounts of cane sugar and MSG to 6% and 1%, respectively (C); formula C...
Tipo: Journal article Palavras-chave: Functional fermented plant beverage; γ-aminobutyric acid; Lactobacillus plantarum; Monosodium glutamate; Red seaweed; Response surface methodology.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300001
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