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| Champagne,Claude P; Gardner,Nancy J; Lacroix,Christophe. |
| The exopolysaccharide (EPS)-producing cultures such as Lactobacillus rhamnosus RW-9595M present a challenge for the culture producers because the high viscosity of the fermented growth medium makes it difficult to recover the cells by centrifugation or filtration. This study examined four approaches to reduce viscosity of the medium while producing high cell densities: incubation temperature, extended incubation in the stationary growth phase, production in alginate gel beads and fed-batch fermentation technology. Automated spectrophotometry (AS) was used to study the effects of temperature, pH and lactate level on growth of the strain. In AS assays, there was no significant difference in final maximal biomass production at temperatures ranging between... |
| Tipo: Journal article |
Palavras-chave: Alginate beads; Fed-batch culture; Temperature. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200005 |
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| Champagne,Claude P; Gaudreau,Hélène; Conway,John. |
| Three brewers and three bakers yeast extracts (YE) were obtained from five commercial suppliers. They were added to microbiological media and their growth-promoting properties were examined using four lactic cultures (Lactobacillus casei EQ28 and EQ85, Lactobacillus acidophilus EQ57, Pediococcus acidilactici MA18/5-M). Bakers YE have a higher total nitrogen content than brewers YE, but there was not always a correlation between the nitrogen content and growth. A systematic preference for bakers YE over brewers YE was only encountered with Lb. casei EQ85, but the other lactic cultures had variable reactions to the source of YE. With Lb. casei EQ85 and Pc. acidilactici 17/5M, mixing of the two sources of YE gave progressively higher growth as a... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300004 |
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