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| Vincent-hubert, Francoise; Chatel, Amelie; Gourlay-france, Catherine. |
| We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase... |
| Tipo: Text |
Palavras-chave: Benzo[a]pyrene; Cadmium; Metallothionein; Aryl hydrocarbon receptor; Antioxidant enzymes; RT-qPCR. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29547/27873.pdf |
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| Chatel, Amelie; Faucet-marquis, Virginie; Pfohl-leszkowicz, Annie; Gourlay-france, Catherine; Vincent Hubert, Francoise. |
| Derived polycyclic aromatic hydrocarbons (PAHs) such as nitro-PAHs are present in the environment and are known to be much more toxic than PAHs compounds. However, very few studies have analysed their effects on the aquatic environment and none have investigated the freshwater environment. In the present study, we determined whether 1-nitropyrene (1-NP), a model of nitro-PAHs, can induce DNA adducts in gills and digestive glands of the freshwater mussel Dreissena polymorpha. Two concentrations of 1-NP (50 and 500 mu M) were tested. In addition, in order to understand the metabolic pathways involved in 1-NP genotoxicity, mRNA expression of genes implicated in biotransformation mechanisms was assessed by quantitative reverse transcription-PCR. Results showed... |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00287/39799/41532.pdf |
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| Chatel, Amelie; Faucet-marquis, Virginie; Perret, Marine; Gourlay-france, Catherine; Uher, Emmanuelle; Pfohl-leszkowicz, Annie; Vincent-hubert, Francoise. |
| The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The objective of the present study is to determine the stability of DNA adducts induced by benzo[a]pyrene (B[a]P) in zebra mussels. Mussels were exposed to dissolved B[a]P (10100 mu g/l) for 4 days. Afterwards, mussels were kept in clean water for 28 days and DNA adduct levels were subsequently measured in two different organs, the digestive glands and the gills, using the P-32-postlabelling technique. In parallel, the expression of genes... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00184/29566/27912.pdf |
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