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	Provedor de dados Genet. Mol. Biol. (2) Autor Chaves,Paulo B. (2) Fagundes,Valéria (2) Mendes,Sérgio L. (2) Paes,Marcela F. (2) Strier,Karen B. (2) Boubli,Jean P. (1) Louro,Iúri D. (1) Possamai,Carla de B. (1) Mais... Palavra-chave Brachyteles (2) Conservation genetics (2) Fecal DNA (2) Endangered species (1) MtDNA (1) Noninvasive sampling (1) PCR-RFLP (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2008 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction Genet. Mol. Biol. Chaves,Paulo B.; Paes,Marcela F.; Mendes,Sérgio L.; Strier,Karen B.; Louro,Iúri D.; Fagundes,Valéria. The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies. Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachyteles; Conservation genetics; Endangered species; Fecal DNA; Noninvasive sampling. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400028 Genetic structure in two northern muriqui populations (Brachyteles hypoxanthus, Primates, Atelidae) as inferred from fecal DNA Genet. Mol. Biol. Fagundes,Valéria; Paes,Marcela F.; Chaves,Paulo B.; Mendes,Sérgio L.; Possamai,Carla de B.; Boubli,Jean P.; Strier,Karen B.. We assessed the genetic diversity of two northern muriqui (Brachyteles hypoxanthus Primata, Atelidae) populations, the Feliciano Miguel Abdala population (FMA, n = 108) in the Brazilian state of Minas Gerais (19°44' S, 41°49' W) and the Santa Maria de Jetibá population (SMJ, n = 18) in the Brazilian state of Espírito Santo (20°01' S, 40°44' W). Fecal DNA was isolated and PCR-RFLP analysis used to analyze 2160 bp of mitochondrial DNA, made up of an 820 bp segment of the gene cytochrome c oxidase subunit 2 (cox2, EC 1.9.3.1), an 880 bp segment of the gene cytochrome b (cytb, EC 1.10.2.2) and 460 bp of the hypervariable segment of the mtDNA control region (HVRI). The cox2 and cytb sequences were monomorphic within and between populations whereas the HVRI... Tipo: Info:eu-repo/semantics/other Palavras-chave: Brachyteles; Conservation genetics; Fecal DNA; MtDNA; PCR-RFLP. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100028 Registros recuperados: 2 Primeira ... 1 ... Última

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