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Partial purification and characterization of xylanase produced by Penicillium expansum BABT
Querido,André Luiz de Souza; Coelho,Jorge Luiz Cavalcante; Araújo,Elza Fernandes de; Chaves-Alves,Virgínia Maria.
An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mumol min-1 mug -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partial purification; Xylan/xylanases; Penicillium expansum.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400016
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Thermostability of xylanolytic enzymes produced by Lentinula edodes UFV70 BJM
Ribeiro,Liliane Fraga Costa; Vaz,Marcelo Gomes Marçal Vieira; Chaves-Alves,Virgínia Maria; Vanetti,Maria Cristina Dantas; Kasuya,Maria Catarina Megumi; Passos,Flávia Maria Lopes; Nascimento,Antônio Galvão do.
Xylanolytic enzymes produced by Lentinula edodes UFV70, cultivated in eucalyptus sawdust/rice bran medium, were stable at 50, 60 and 65ºC for 21 hours, losing only 15-25% activity. Fungus incubation at 50ºC for 12 hours and at 65ºC for 24 hours increased the amount of xylose produced.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Thermostability; Lentinula edodes; Xylose.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100021
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Screening of filamentous fungi for production of xylitol from D-xylose BJM
Sampaio,Fábio Coelho; Silveira,Wendel Batista da; Chaves-Alves,Virgínia Maria; Passos,Flávia Maria Lopes; Coelho,Jorge Luiz Cavalcante.
Eleven filamentous fungi were screened for xylitol production in batch cultures. Production was generally low under the growth conditions used in this study. Penicillium crustosum presented the highest production, 0.52 g L-1 from 11.50 g L-1 of D-xylose, representing consumption of 76% of the original D-xylose.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Xylose; Bioconversion; Xylitol; Filamentous fungi.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400007
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