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| Registros recuperados: 17 | |
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| Hardison, D. Ransom; Holland, William C.; Mccall, Jennifer R.; Bourdelais, Andrea J.; Baden, Daniel G.; Darius, H. Taiana; Chinain, Mireille; Tester, Patricia A.; Shea, Damian; Quintana, Harold A. Flores; Morris, James A., Jr.; Litaker, R. Wayne. |
| Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA((R))). However, the license requirements and regulations pertaining to radioisotope utilization can limit the applicability of the RBA((R)) in certain labs. A fluorescence based receptor binding assay (RBA((F))) was developed to provide an alternative method of screening fish samples for CTXs in facilities not certified to use radioisotopes. The new assay is based on competition binding between CTXs and fluorescently labeled brevetoxin-2 (BODIPY (R)-PbTx-2) for voltage-gated sodium channel receptors at site 5 instead... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00664/77575/79555.pdf |
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| Kerbrat, Anne Sophie; Amzil, Zouher; Pawlowiez, Ralph; Golubic, Stjepko; Sibat, Manoella; Darius, Helene Taiana; Chinain, Mireille; Laurent, Dominique. |
| Marine pelagic diazotrophic cyanobacteria of the genus Trichodesmium (Oscillatoriales) are widespread throughout the tropics and subtropics, and are particularly common in the waters of New Caledonia. Blooms of Trichodesmium are suspected to be a potential source of toxins in the ciguatera food chain and were previously reported to contain several types of paralyzing toxins. The toxicity of water-soluble extracts of Trichodesmium spp. were analyzed by mouse bioassay and Neuroblastoma assay and their toxic compounds characterized using liquid chromatography coupled with tandem mass spectrometry techniques. Here, we report the first identification of palytoxin and one of its derivatives, 42-hydroxy-palytoxin, in field samples of Trichodesmium collected in... |
| Tipo: Text |
Palavras-chave: Cyanobacteria; Trichodesmium; Palytoxin; 42-hydroxy-palytoxin; Clupeotoxism. |
| Ano: 2011 |
URL: https://archimer.ifremer.fr/doc/00040/15143/12576.pdf |
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| Gatti, Clemence Mahana Iti; Lonati, Davide; Darius, Helene Taiana; Zancan, Arturo; Roue, Melanie; Schicchi, Azzurra; Locatelli, Carlo Alessandro; Chinain, Mireille. |
| Ciguatera fish poisoning (CFP) is the most prevalent non-bacterial food-borne form of poisoning in French Polynesia, which results from the consumption of coral reef fish naturally contaminated with ciguatoxins produced by dinoflagellates in the genus Gambierdiscus. Since the early 2000s, this French territory has also witnessed the emergence of atypical forms of ciguatera, known as ciguatera shellfish poisoning (CSP), associated with the consumption of marine invertebrates. In June 2014, nine tourists simultaneously developed a major and persistent poisoning syndrome following the consumption of the gastropod Tectus niloticus collected in Anaho, a secluded bay of Nuku Hiva Island (Marquesas Archipelago, French Polynesia). The unusual nature and severity... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Tectus niloticus; Ciguatoxins; Health hazards; Clinical follow-up; Neurological exploration; French Polynesia. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00660/77228/78664.pdf |
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| Hardison, D. Ransom; Holland, William C.; Darius, H. Taiana; Chinain, Mireille; Tester, Patricia A.; Shea, Damian; Bogdanoff, Alex K.; Morris, James A., Jr.; Quintana, Harold A. Flores; Loeffler, Christopher R.; Buddo, Dayne; Litaker, R. Wayne. |
| Lionfish, native to reef ecosystems of the tropical and sub-tropical Indo-Pacific, were introduced to Florida waters in the 1980s, and have spread rapidly throughout the northwestern Atlantic, Caribbean Sea and the Gulf of Mexico. These invasive, carnivorous fish significantly reduce other fish and benthic invertebrate biomass, fish recruitment, and species richness in reef ecosystems. Fisheries resource managers have proposed the establishment of a commercial fishery to reduce lionfish populations and mitigate adverse effects on reef communities. The potential for a commercial fishery for lionfish is the primary reason to identify locations where lionfish accumulate sufficient amounts of ciguatoxin (CTX) to cause ciguatera fish poisoning (CFP), the... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00660/77221/78672.pdf |
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| Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille. |
| The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tripneustes gratilla; Sea urchin; Echinoidea; Gambierdiscus polynesiensis; Windowscreens; Artificial substrates; QPCR assays; CBA-N2a; LC-MS/MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00436/54716/56153.pdf |
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| Longo, Sébastien; Sibat, Manoella; Taiana Darius, Hélène; Hess, Philipp; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. Ciguatera outbreaks are expected to increase worldwide with global change, in particular as a function of its main drivers, including changes in sea surface temperature, acidification, and coastal eutrophication. In French Polynesia, G. polynesiensis is regarded as the dominant source of CTXs entering the food web. The effects of pH (8.4, 8.2, and 7.9), Nitrogen:Phosphorus ratios (24N:1P vs. 48N:1P), and nitrogen source (nitrates vs. urea) on growth rate, biomass, CTX levels, and profiles were examined in four clones of G. polynesiensis at different culture age (D10, D21,... |
| Tipo: Text |
Palavras-chave: Gambierdiscus polynesiensis; French Polynesia; Ciguatera; Ciguatoxins; LC-MS; MS; Toxin profile; Nitrate; Urea; Culture medium acidification. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77382/78981.pdf |
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| Roué, Mélanie; Smith, Kirsty F.; Sibat, Manoella; Viallon, Jérôme; Henry, Kévin; Ung, André; Biessy, Laura; Hess, Philipp; Darius, Hélène Taiana; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed... |
| Tipo: Text |
Palavras-chave: Ciguatera monitoring; Gambierdiscus; Ciguatoxins; SPATT passive samplers; HP20 resin; CBA-N2a; LC-MS; MS; WS artificial substrate; QPCR; HTS metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74143/73670.pdf |
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| Roue, Melanie; Darius, Helene Taiana; Ung, Andre; Viallon, Jerome; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Chinain, Mireille. |
| Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were... |
| Tipo: Text |
Palavras-chave: Giant clams; Ex situ exposure to toxic algae; Ciguatoxins; Gambierdiscus polynesiensis; Anatomical distribution; Toxin elimination; CBA-N2a; LC-MS/MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00440/55123/56579.pdf |
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| Viallon, Jerome; Chinain, Mireille; Darius, Helene Taiana. |
| The neuroblastoma cell-based assay (CBA-N2a) is widely used for the detection of marine biotoxins in seafood products, yet a consensus protocol is still lacking. In this study, six key parameters of CBA-N2a were revisited: cell seeding densities, cell layer viability after 26 h growth, MTT incubation time, Ouabain and Veratridine treatment and solvent and matrix effects. A step-by-step protocol was defined identifying five viability controls for the validation of CBA-N2a results. Specific detection of two voltage gated sodium channel activators, pacific ciguatoxin (P-CTX3C) and brevetoxin (PbTx3) and two inhibitors, saxitoxin (STX) and decarbamoylsaxitoxin (dc-STX) was achieved, with EC(50)values of 1.7 +/- 0.35 pg/mL, 5.8 +/- 0.9 ng/mL, 3 +/- 0.5 ng/mL... |
| Tipo: Text |
Palavras-chave: CBA-N2a; Standardization; Matrix effects; Absorbance data; Ciguatoxins; Brevetoxins; Saxitoxins; Biological sample; Seafood safety. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76859/78083.pdf |
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| Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille. |
| Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tectus niloticus; Gastropods; Gambierdiscus polynesiensis; QPCR assays; CBA-N2a; LC-MS; MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00426/53798/54719.pdf |
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| Chomérat, Nicolas; Bilien, Gwenael; Derrien, Amelie; Henry, Kévin; Ung, André; Viallon, Jérôme; Darius, Hélène Taiana; Mahana Iti Gatti, Clémence; Roué, Mélanie; Hervé, Fabienne; Réveillon, Damien; Amzil, Zouher; Chinain, Mireille. |
| To date, the genus Ostreopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. The taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. The species Ostreopsis lenticularis was first described by Y. Fukuyo from French Polynesia using light microscopy observations, but without genetic information associated. The present study aims at revisiting the morphology, molecular phylogeny and toxicity of O. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of French... |
| Tipo: Text |
Palavras-chave: ITS-5.8S rDNA; LSU rDNA; Microscopy; Ostreopsis lenticularis; Taxonomy; Toxins. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00486/59747/62887.pdf |
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| Longo, Sebastien; Sibat, Manoella; Viallon, Jérôme; Darius, Hélène Taiana; Hess, Philipp; Chinain, Mireille. |
| Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds,... |
| Tipo: Text |
Palavras-chave: Gambierdiscus polynesiensis; Ciguatera; Ciguatoxins; Gambierone; 44-methylgambierone; LC-MS/MS; CBA-N2a; Toxin profiles. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00599/71134/69449.pdf |
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| Clausing, Rachel J.; Losen, Barbara; Oberhaensli, Francois R.; Darius, H. Taiana; Sibat, Manoella; Hess, Philipp; Swarzenski, Peter W.; Chinain, Mireille; Bottein, Marie-yasmine Dechraoui. |
| Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g.1 fish daily, 0.4 ¦Ìg CTX3C equiv kg.1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ¡À 0.2 ¦Ìg CTX3C equiv kg.1). Although tissue CTX concentrations stabilized after 8 weeks (¡«3 ¡À 0.5 ¦Ìg CTX3C equiv kg.1), muscle toxin burden (total ¦Ìg CTX in muscle tissue)... |
| Tipo: Text |
Palavras-chave: Ciguatoxin; Bioaccumulation; Growth dilution; Trophic transfer; Herbivorous fish; Experimental model. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00442/55339/60476.pdf |
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| Van Dolah, Frances M.; Morey, Jeanine S.; Milne, Shard; Ung, Andre; Anderson, Paul E.; Chinain, Mireille. |
| Marine dinoflagellates produce a diversity of polyketide toxins that are accumulated in marine food webs and are responsible for a variety of seafood poisonings. Reef-associated dinoflagellates of the genus Gambierdiscus produce toxins responsible for ciguatera poisoning (CP), which causes over 50,000 cases of illness annually worldwide. The biosynthetic machinery for dinoflagellate polyketides remains poorly understood. Recent transcriptomic and genomic sequencing projects have revealed the presence of Type I modular polyketide synthases in dinoflagellates, as well as a plethora of single domain transcripts with Type I sequence homology. The current transcriptome analysis compares polyketide synthase (PKS) gene transcripts expressed in two species of... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00657/76863/78096.pdf |
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| Chinain, Mireille; Mahana Iti Gatti, Clémence; Ung, André; Cruchet, Philippe; Revel, Taina; Viallon, Jérôme; Sibat, Manoella; Varney, Patrick; Laurent, Victoire; Hess, Philipp; Taiana Darius, Hélène. |
| Ciguatera poisoning (CP) results from the consumption of seafood contaminated with ciguatoxins (CTXs). This disease is highly prevalent in French Polynesia with several well-identified hotspots. Rapa Island, the southernmost inhabited island in the country, was reportedly free of CP until 2007. This study describes the integrated approach used to investigate the etiology of a fatal mass-poisoning outbreak that occurred in Rapa in 2009. Symptoms reported in patients were evocative of ciguatera. Several Gambierdiscus field samples collected from benthic assemblages tested positive by the receptor binding assay (RBA). Additionally, the toxicity screening of ≈250 fish by RBA indicated ≈78% of fish could contain CTXs. The presence of CTXs in fish was confirmed... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; French Polynesia; Gambierdiscus; Ciguatoxins; Epidemiology; Toxicological analyses; Risk management; Climate change. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77362/78933.pdf |
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| Roue, Melanie; Darius, Helene Taiana; Picot, Sandy; Ung, Andre; Viallon, Jerome; Gaertner-mazouni, Nabila; Sibat, Manoella; Amzil, Zouher; Chinain, Mireille. |
| Ciguatera Fish Poisoning (CFP) is a foodborne disease classically related to the consumption of tropical coral reef fishes contaminated with ciguatoxins (CTXs), neurotoxins produced by dinoflagellates of the Gambierdiscus genus. Severe atypical ciguatera-like incidents involving giant clams, a marine resource highly consumed in the South Pacific, are also frequently reported in many Pacific Islands Countries and Territories. The present study was designed to assess the ability of giant clams to accumulate CTXs in their tissues and highlight the potential health risks associated with their consumption. Since giant clams are likely to be exposed to both free-swimming Gambierdiscus cells and dissolved CTXs in natural environment, ex situ contamination... |
| Tipo: Text |
Palavras-chave: Giant clams; Ex situ contamination; Ciguatoxins; Gambierdiscus polynesiensis; LC-MS/MS; Neuroblastoma cell-based assay. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00343/45415/45123.pdf |
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| Registros recuperados: 17 | |
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