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Gametogenetic cycle and reproductive effort assessed by two methods in 3 age classes of Pacific oysters, Crassostrea gigas, reared in Normandy ArchiMer
Royer, J; Seguineau, Catherine; Park, K; Pouvreau, Stephane; Choi, K; Costil, K.
Two methods were used to estimate the reproductive output of female Pacific oysters reared in Normandy: histology with image analysis and ELISA (Enzyme-Linked lmmunoSorbent Assay) which allowed the quantification of egg protein. Condition indices, gonad area and gametogenetic stages of the oysters were determined in the entire population (males and females) between May and October 2005. All investigations were performed in 3 age classes: oysters in their first, second or third years (corresponding to spat, half-grown and market-sized oysters, respectively). Both quantitative histology and ELISA provided similar results in terms of reproductive effort (illustrated by the gonado-somatic index, GSI) except during the GSI drop, corresponding to spawning, which...
Tipo: Text Palavras-chave: Reproductive effort; Image analysis; Gametogenetic cycle; ELISA; Crassostrea gigas; Condition index.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4305.pdf
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