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| Registros recuperados: 97 | |
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| Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence. |
| Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in... |
| Tipo: Text |
Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf |
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| Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Goubet, Anne; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Costantini, Louis; Masson, Jean-claude; Martin, Anne-genevieve. |
| Created in 1986, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91 /67/EEC and 95/70/EC and are a patt of the institutional tasks ofIfremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to survey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Banc de Granville and zone X). In 2004, no sample couId be analysed to these two areas because of the difficulty to obtain flat oysters (sparse oyster beds,... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00086/19702/17343.pdf |
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| Morga, Benjamin; Faury, Nicole; Guesdon, Stephane; Chollet, Bruno; Renault, Tristan. |
| Since 2008, mass mortality outbreaks associated with the detection of particular variants of OsHV-1 have been reported in Crassostrea gigas spat and juveniles in several countries. Recent studies have reported information on viral replication during experimental infection. Viral DNA and RNA were also detected in the haemolymph and haemocytes suggesting that the virus could circulate through the circulatory system. However, it is unknown if the virus is free in the haemolymph, passively associated at the surface of haemocytes, or able to infect and replicate inside these cells inducing (or not) virion production. In the present study, we collected haemocytes from the haemolymphatic sinus of the adductor muscle of healthy C. gigas spat and exposed them in... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00399/50997/51780.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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| Berthe, Franck; Pichot, Yves; Chollet, Bruno; Thebault, Anne; Kuntz, Gregoire; Tige, Gilbert; Thebault, Anne; Fleury, Pierre-gildas; Hirata, Toshio; Le Coguic, Yvette; Renault, Tristan; Lipart, Cecile; Grizel, Henri; Joly, Jean-pierre; Mazurie, Joseph; Baud, Jean-pierre; Goulletquer, Philippe; Dreno, Jean-paul; Buestel, Dominique. |
| Le réseau de veille zoosanitaire des mollusques, REPAMO, a été créé en 1992 afin de protéger les espèces d'intérêt économique des maladies susceptibles de les affecter. En effet, de même que les autres productions animales, la conchyliculture est frappée par diverses maladies dont certaines se comportent comme des facteurs limitants de la qualité ou de la quantité des productions. L' histoire de l'ostréiculture française a connu de tristes expériences en la matière, notamment dans les années 70 pour l' huître creuse portugaise, Crassostrea angulata ou encore pour l'huître plate, Ostrea edulis. En fait, nous ne disposons que de très peu de possibilités de protéger les mollusques bivalves vis à vis des maladies infectieuses. D' une part, les traitements,... |
| Tipo: Text |
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00098/20901/18521.pdf |
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| Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit. |
| Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie.... |
| Tipo: Text |
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf |
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| Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe. |
| Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En... |
| Tipo: Text |
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf |
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| Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno. |
| Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés. |
| Tipo: Text |
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf |
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| Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Guilpain, Patrice; Soletchnik, Patrick; Faury, Nicole; Seugnet, Jean-luc; Arnaud, Christophe; Chollet, Bruno; Chabirand, Jean-michel; Fleury, Pierre-gildas; Langlade, Aime; Goulletquer, Philippe; Mille, Dominique. |
| Le Bassin ostréicole de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1986), par un net recul des rendements de production qui engendrent une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est faite depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation défavorable pour la majorité des zones charentaises en terme de croissance-production est annuellement confirmée, depuis 1993 au moyen des réseaux de croissance... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Eaux profondes; Crassostrea gigas; Technique élevage; Croissance; Mortalité; Biométrie; Biochimie; Marennes Oléron. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00072/18369/15940.pdf |
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| Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle. |
| Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of <1% and a minimum detection limit of 50 gene copies per reaction. Using... |
| Tipo: Text |
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf |
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| Garcia, Celine; Haond, Christophe; Chollet, Bruno; Nerac, Mirella; Omnes, Emmanuelle; Joly, Jean-pierre; Dubreuil, Christine; Serpin, Delphine; Langlade, Aime; Le Gal, Dominique; Terre-terrillon, Aouregan; Courtois, Olivier; Guichard, Benjamin; Arzul, Isabelle. |
| Background: Microcell parasites are small intracellular protozoans mostly detected in molluscs and can be associated with mortalities. In 2010 and 2011, strong increases in mortality events were reported in different wild beds of the wedge clam Donax trunculus Linnaeus, along the Atlantic coast of France and the presence of potential pathogens, including microcells, was investigated. Methods: Clams collected in different beds showing mortality were examined by histology. Based on histological observations, confirmatory analyses were carried out, including transmission electron microscopy (TEM) and molecular characterization. Results: Histological analyses revealed the presence of small protozoans similar to microcell parasites in different tissues of Donax... |
| Tipo: Text |
Palavras-chave: Mikrocytos veneroides n. sp.; Mikrocytos donaxi n. sp.; Donax trunculus; Mortality; Microcell parasite. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00428/53989/55149.pdf |
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| Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno. |
| Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared at 3 different locations. A survey was taken of these young C. virginica oysters during 1993. Histological and electron microscopical examination revealed a parasite interpreted as a Martelia-like protozoan in 1 oyster. The structure and the initial stages of the developmental cycle of this parasite are described for the first time in American oysters. However, no mature spores were detected. Thus, exact identification of the protozoan is difficult. This study indicates that American oysters could be infected with Marteilia spp. during cohabitation with Marteilia-infected native bivalves in France. |
| Tipo: Text |
Palavras-chave: Marteilia like parasite; Marteiliosis; Crassostrea virginica; American oyster. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/1995/publication-2897.pdf |
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| Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin. |
| The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members... |
| Tipo: Text |
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf |
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| Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle. |
| The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses.... |
| Tipo: Text |
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf |
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| Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
| Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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| Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Guilpain, Patrice; Geairon, Philippe; Arnaud, Christophe; Chollet, Bruno; Faury, Nicole; Soletchnik, Patrick; Seugnet, Jean-luc; Mille, Dominique. |
| Le Bassin ostréicole de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1986), par un net recul des rendements de production qui engendre une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est démontrée depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation défavorable pour la majorité des zones charentaises en terme de « croissance - production» est annuellement confirmée, depuis 1993 au moyen des réseaux de suivi de... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Elevage en eaux profondes; Suivis expérimentaux; Biométrie; Mortalité; Bassin de Marennes Oléron; Fouras; Saumonards. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00134/24525/22559.pdf |
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| Registros recuperados: 97 | |
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