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| Søltoft, Malene; Christensen, J.H.; Nielsen, J.; Knuthsen, Pia. |
| A rapid and reliable analytical method for quantification of flavonoids in onions was developed and validated. Five extraction methods were tested on freeze-dried onions and subsequently high performance liquid chromatography (HPLC) with UV detection was used for quantification of seven flavonoids. The extraction efficiencies were lowest for the conventional water bath extraction compared to pressurized liquid extraction (PLE), ultrasonication, ultrasonic liquid processor, and microwave extraction, which yielded similar efficiencies. The reproducibility was in the same range (RSD: 1–11%) for all tested extraction methods. However, PLE was the preferred extraction method because the method can be highly automated, use only small amounts of solvents,... |
| Tipo: Journal paper |
Palavras-chave: Research methodology and philosophy. |
| Ano: 2009 |
URL: http://orgprints.org/19117/4/19117.pdf |
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