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Registros recuperados: 39 | |
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Hui, Belinda; Demoy-schneider, Marina; Vonau, Vincent; Le Moullac, Gilles; Moriceau, Jacques; Le Pennec, Marcel; Cochard, Jean-claude. |
Cryopreservation is a useful tool for genetic improvement which has been applied to several bivalve mollusk species. It would allow to keep the gametes of selected individuals and to preserve ex-situ the biodiversity of wild populations of the black-lip pearl oyster Pinctada margaritifera threatened of standardization by the seed transfers between the islands in French Polynesia. The ability to cryopreserve spermatozoa would bring about significant benefits to the cultured black pearl industry. Sperm freezing supposes the control of different steps: preparation of breeders, sperm collection, evaluation of sperm quality and the freezing process itself. Broodstock conditioning in hatchery allows to obtain gametes from the pearl oyster. Sperm quality is... |
Tipo: Text |
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Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14043/11238.pdf |
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Cochard, Jean-claude. |
La production de naissain de Pecten maximus destiné au repeuplement de la rade de Brest a nécessité le déclenchement de la ponte de plusieurs centaines d'individus au cours des années 1980 à 1984. L'analyse de ces données permet de faire quelques constatations sur le cycle sexuel de la coquille Saint-Jacques dans cette zone. - Le cycle sexuel de la population est très étalé dans le temps. Des pontes d'apparence normale peuvent être obtenues chaque mois sans qu'il soit nécessaire de recourir au conditionnement. - La fécondité, estimée par le nombre moyen d'ovocytes recueillis par reproducteur, varie au cours du cycle annuel. Les pontes les plus abondantes ont lieu de février à avril (12 à 20 millions d'ovocytes par coquille en moyenne) et en octobre. En été... |
Tipo: Text |
Palavras-chave: Ovocytes; Pecten maximus; Coquille Saint Jacques; Reproduction; Naissain. |
Ano: 1985 |
URL: http://archimer.ifremer.fr/doc/1985/acte-6540.pdf |
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Fournier, Jonathan; Dupuy, Celie; Bouvy, Marc; Couraudon-reale, Marine; Charpy, Loic; Pouvreau, Stephane; Le Moullac, Gilles; Le Pennec, Marie-annick; Cochard, Jean-claude. |
In atoll lagoons of French Polynesia, growth and reproduction of pearl oysters are mainly driven by plankton concentration. However, the actual diet of black-lip pearl oysters P. margaritifera in these lagoons is poorly known. To fill this gap, we used the flow through chamber method to measure clearance rates of P. margaritifera in Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia). We found : (i) that pearl oysters cleared plankton at a rate that was positively related to plankton biovolume, (ii) that nanoflagellates were the main source of carbon for the pearl oysters, (iii) that the quantity and origin of carbon filtrated by pearl oysters was highly dependent on the concentration and composition of plankton. These results provide essential... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Clearance rates; French Polynesia; Phytoplankton; Protists. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00078/18888/16599.pdf |
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Linard, Clementine; Gueguen, Yannick; Moriceau, Jacques; Soyez, Claude; Hui, Belinda; Raoux, Aurore; Cuif, Jean Pierre; Cochard, Jean-claude; Le Pennec, Marcel; Le Moullac, Gilles. |
Marine mollusc shell growth has been widely measured using fluorochrome marking. In order to test the efficiency and reliability of calcein staining on P. margaritifera shells and pearls, the present study examined two administration methods, different concentrations and several immersion times.Immersion in a 150 mg.L-1 calcein solution for 12 h to 24 h appeared to be the best method for marking P. margaritifera shells. For pearl marking, injection of a 200 mg.L-1 calcein solution into the pearl pouch was the optimal method. Calcein marking was then used to measure the influence of food resource levels on the shell growth. Groups of 23-month-old P. margaritifera were fed at three trophic levels for two months. The two highest food levels tested (6 000... |
Tipo: Text |
Palavras-chave: Bivalvia; Pearl; Shell; Growth; Scanning electron microscope. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00028/13907/11195.pdf |
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Buestel, Dominique; Nicolas, R.; Morize, E.; Rohan, R.; Latrouite, Daniel; Le Borgne, Y.; Cochard, Jean-claude; Flassch, Jean-pierre; Uno, Yutaka; Audouin, Jacques; Legall, J.y.; Jezequel, M.; Lorec, J.; Henocque, Yves; Reveche, C.; Girin, Michel; Laurec, Alain; Herry, P.. |
L'aquaculture extensive et le repeuplement sont deux modes d'exploitation des ressources halieutiques qui ne font appel, une fois les juvéniles produits, ni à un apport de nourriture artificielle, ni à un contrôle continu de la part de l'exploitant, mais dépendent par contre de la productivité du milieu marin, Cette approche repose sur l'idée que le principal facteur limitant d'une population naturelle est le faible nombre de survivants après les premiers stades, et que l'on peut par alevinage, aménagement du milieu ou autres techniques, augmenter la production d'une espèce exploitable, Le but de ces journées d'étude est de faire le point des connaissances sur ces sujets, à l'intention des scientifiques mais aussi des utilisateurs, Il a paru souhaitable de... |
Tipo: Text |
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Ano: 1979 |
URL: http://archimer.ifremer.fr/doc/00018/12941/9907.pdf |
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Lemonnier, Hugues; Bernard, E; Boglio, E; Goarant, Cyrille; Cochard, Jean-claude. |
Penaeid shrimp reared in earthen ponds are exposed to sediment, which can, in some instances, induce a stress. In seawater, the osmoregulatory capacity (hyporegulation) is a useful tool to compare the physiological condition of shrimp exposed to various stressors. By keeping some shrimp in cages at different locations of a single pond heterogeneous in terms of sediment quality, it was possible, using osmotic pressure (OP), to identify some locations where the stress was maximum and some others where it was minimum. Simultaneously, sediment samples were taken and analysed in order to evaluate some physico-chemical parameters that could be related to the stress observed in the shrimps kept in the cages. This approach allowed to show a significant positive... |
Tipo: Text |
Palavras-chave: PH; Sediments; Stress; Osmotic pressure; Shrimp physiology. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-1866.pdf |
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Le Moullac, Gilles; Hui, Belinda; Vonau, Vincent; Levy, Peva; Cochard, Jean-claude. |
Preliminary experiments allowed defining basic requirements of Pinctada margaritifera for somatic and gonadic growth in controlled conditions. This study was continued by observation of the gametogenesis at a cellular level. In April/May 2008, 125 oysters were conditioned for 30 days in 25 tubular tanks (30 L). Water was renewed 4 times/hour. A mixed (v:v) diet of Isochrysis galbana (T-Iso) and Chaetoceros gracilis was supplied continuously. A control batch of 125 oysters was maintained in trays in the lagoon. An initial sample of 30 individuals was made. Gonadic changes were first characterized using a gonadal development index (GDI) based on the ratio of the gonad area to the total visceral mass area on a sagittal section of the body. Histological... |
Tipo: Text |
Palavras-chave: Pearl oyster; Reproduction; Gametogenesis; Broodstock. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14010/11196.pdf |
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Pouvreau, Stephane; Rambeau, Marc; Cochard, Jean-claude; Robert, Rene. |
Classically, investigation of the internal morphology and composition of molluscs and especially bivalves relies on destructive method (biometry, biochemistry and histology). These techniques have given essential information, but in contrast are time consuming and lead to the irreversible loss of the animal while they don't allow integration of the various levels of molecular-to-organism functioning. The aim of this study is to analyze for the first time the potential of NMR (nuclear magnetic resonance) imaging (MRI) to depict, with sufficient resolution and satisfactory contrast, the anatomy of a bivalve model, the Pacific oyster, Crassostrea gigas, without opening it. MRI experiments were carried out at 19 °C in several non-anaesthetized adult Pacific... |
Tipo: Text |
Palavras-chave: In vivo physiology; Aquaculture; Anatomy; MRI; Pacific oyster. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1819.pdf |
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Buestel, Dominique; Cochard, Jean-claude; Gerard, Andre. |
Des travaux expérimentaux menés depuis 1977 en rade de Brest ont permis la mise au point d'une méthode de production de naissain de coquille Saint-Jacques dont la fiabilité a été démontrée en 1982. La filière d'élevage comprend quatre phases nécessitant chacune la mise en ouvre de techniques originales sur des sites distincts. La première étape réalisée en écloserie (température 17°C - eau de mer filtrée à 1µ) produit des larves pédiveligères de 250µ. Ces larves reprises dans une nurserie (eau de mer brute filtre à 125µ) donnent des postlarves de 1 à 3 mm. Les deux dernières phases se passent directement en mer ; un premier stade permet de produire des animaux de 10 mm environ qui sont ensuite portés à une taille de 30 mm et plus. Les résultats de l'année... |
Tipo: Text |
Palavras-chave: Pecten maximus; Coquille Saint Jacques; Production artificielle. |
Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/00019/13019/9996.pdf |
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Cochard, Jean-claude. |
Les crevettes pénéides maintenues captives dans des cages posées sur le fond des bassins d'élevage subissent un stress dû au sédiment qui peut être évalué par la comparaison entre la valeur de la pression osmotique de leur hémolymphe et celle des crevettes libres du même bassin. Les objectifs des travaux réalisés à la station de Saint-Vincent (SASV, Nouvelle Calédonie) étaient de confirmer la variabilité spatiale de l'intensité du stress et de rechercher dans les sédiments un indicateur physique ou chimique corrélé à ce stress. Des lots de 25 à 30 crevettes de 13,2g, pêchées à l'épervier, ont été placés dans des cages de grillage métallique déposées en 12 points du bassin d'élevage. Les cages ont été relevées au bout de 24 heures et la pression osmotique... |
Tipo: Text |
Palavras-chave: Penaeus stylirostris; Capacité osmorégulatrice; Stress; Sédiment; Aquaculture; Crustacés. crevettes pénéides; PH. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00131/24265/22263.pdf |
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Le Moullac, Gilles; Soyez, Claude; Sham-koua, Manaarii; Levy, Peva; Moriceau, Jacques; Vonau, Vincent; Maihota, Mayalen; Cochard, Jean-claude. |
This study aimed to model the food intake of P. margaritifera in order to examine the relationship between food level and reproductive activity. The effect of microalgae concentration on ingestion rate and assimilation efficiency was studied over a broad concentration range, using a mixture of Isochrysis galbana and Chaetoceros gracilis. Reproductive effort was assessed using three microalgae concentrations of 0.5, 7 and 18 cell μL-1. Reproductive status was assessed by gonad development index (GDI) – the ratio of the gonad surface to the visceral mass surface – and histological analysis of the gonad based on the presence (continuous or discontinuous) or the absence of gonial cells (GC). Ingestion is a saturating function of seston concentration for... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Food level; Ingestion rate; Reproduction; Gametogenesis. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00050/16085/13562.pdf |
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Soyez, Claude; Tiapari, Jerome; Teissier, Hinano; Cochard, Jean-claude. |
En Polynésie française, la perliculture s'est développée à partir de naissain de captage. Les transferts de juvéniles entre les îles ont conduit à une certaine uniformisation des populations mais l'huître perlière reste essentiellement un animal sauvage (Arnaud et al. ,2003). Une des voies d'amélioration de la qualité des perles et de la rentabilité de la perliculture repose sur la sélection d'individus intéressants et/ou plus performants en particulier comme donneurs de greffons (couleur). L'étape qui consiste à multiplier les animaux sélectionnés passe nécessairement par le contrôle de la reproduction. Les travaux menés dans le cadre de précédentes conventions sur la triploïdisation, la cryoconservation et la maturation ovocytaire (Te Reko Parau N°16 et... |
Tipo: Text |
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Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00304/41517/40725.pdf |
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Le Moullac, Gilles; Tiapari, Jerome; Teissier, Hinano; Martinez, Elodie; Cochard, Jean-claude. |
The growth and reproductive cycle of cultured black-lipped pearl oysters, Pinctada margaritifera (L.), were studied in the Gambier Islands (134°52' W, 23°07' S) from September 2002 to August 2003. Temperatures were recorded throughout the year, revealing seasonal temperature variations between 22.3 °C and 27.8 °C. The mean annual chlorophyll a value, as computed from satellite data, was 0.188 ± 0.075 µg L-1. To study growth and reproduction, 720 two-year-old individuals were ear hung on longlines suspended at a depth of 7 m. Samples were taken twice a month to obtain the following measurements: shell height; wet weight of flesh and total oyster; dry weight of adductor muscle, mantle and visceral mass; and glycogen content. Gonad development was also... |
Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Growth; Reproduction; Temperature; Chlorophyll; Seasonality; Gambier archipelago. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00040/15099/13570.pdf |
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Chevolot, Lionel; Cochard, Jean-claude; Yvin, Jean-claude. |
Delayed metamorphosis usually occurs when scallop Pecten maximus (L.) larvae are reared in still water. Metamorphosis rates may be increased by adding aqueous extracts of certain marine red algae, including one [Delesseria sanguinea (Linne) Lamouroux (Delesseriaceae, Rhodomelaceae)] from which various chemical inducers have been isolated. Of these, jacaranone (a simple quinol) is by far the most active component; other components (p-OH phenylacetic acid methyl ester, p-OH benzaldehyde, p-OH benzyl alcohol) are only weak inducers. All are catabolic derivatives of tyrosine. Homogentisic acid (biosynthetically related to jacaranone) and some catecholamines (L-DOPA, epinephrine) can also induce metamorphosis of P. maximus larvae. All these compounds can be... |
Tipo: Text |
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Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00182/29348/28137.pdf |
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Registros recuperados: 39 | |
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