SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

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	BABT (1)
	BJM (1)
	Rev. Microbiol. (1)

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	Info:eu-repo/semantics/article (2)
	Info:eu-repo/semantics/other (1)

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	1998 (1)

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Partial purification and characterization of xylanase produced by Penicillium expansum

BABT

Querido,André Luiz de Souza; Coelho,Jorge Luiz Cavalcante; Araújo,Elza Fernandes de; Chaves-Alves,Virgínia Maria.

An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mumol min-1 mug -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+.

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Partial purification; Xylan/xylanases; Penicillium expansum.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400016

Screening of filamentous fungi for production of xylitol from D-xylose

BJM

Sampaio,Fábio Coelho; Silveira,Wendel Batista da; Chaves-Alves,Virgínia Maria; Passos,Flávia Maria Lopes; Coelho,Jorge Luiz Cavalcante.

Eleven filamentous fungi were screened for xylitol production in batch cultures. Production was generally low under the growth conditions used in this study. Penicillium crustosum presented the highest production, 0.52 g L-1 from 11.50 g L-1 of D-xylose, representing consumption of 76% of the original D-xylose.

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Xylose; Bioconversion; Xylitol; Filamentous fungi.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400007

Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum

Rev. Microbiol.

Minussi,Rosana Cristina; Soares-Ramos,Juliana Rocha Lopes; Coelho,Jorge Luiz Cavalcante; Silva,Daison Olzany.

The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Pectin lyase; Polygalacturonase; Pectinases; Penicillium griseoroseum; Sugar-cane juice.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400002

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