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| Coelho,Stefânia Vilas Boas; Rosa,Sttela Dellyzete Veiga Franco da; Fantazzini,Tatiana Botelho; Baute,Júlia Lima; Silva,Luciano Coutinho. |
| ABSTRACT Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drying in silica gel; Recalcitrant seeds; Ice crystals; Isoenzymes; Physiological quality. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600588 |
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| Figueiredo,Madeleine Alves de; Rosa,Sttela Dellyzete Veiga Franco da; Ricaldoni,Marcela Andreotti; Pereira,Cristiane Carvalho; Coelho,Stefânia Vilas Boas; Silva,Luciano Coutinho. |
| ABSTRACT Cryopreservation is a technique that may potentially conserve the germplasm of species of the Coffea genus for an indeterminate time. The aim of this study was to evaluate the physiological, biochemical and ultrastructural characteristics of cryopreserved seeds of Coffea arabica L., cultivar Catucaí amarelo IAC 62, which was subjected to different protocols regarding dehydration, precooling, cooling, rewarming and cathode water use. According to each protocol, the seeds were subjected to fast or slow drying to moisture contents of 17 or 20% (wet basis), cooled in different ways, and then immersed in liquid nitrogen for 24 hours. Different rewarming times in a water bath were also used. Physiological, biochemical and ultrastructural analyses were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cathodic water; Dehydration; Long-term conservation; Ultrafast cooling.. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100203 |
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| Caldeira,Carla Massimo; Carvalho,Maria Laene Moreira de; Guimarães,Renato Mendes; Coelho,Stefânia Vilas Boas. |
| RESUMO: O processo de pelotização pode afetar a qualidade das sementes por ocasião da semeadura, por isso, para avaliar o efeito desse processo na qualidade das sementes, foram utilizadas sementes de duas cultivares de tabaco 'CSC 467' e 'CSC 07', coletadas em diferentes etapas do processo de beneficiamento e pelotização: 1) sementes nuas não beneficiadas; 2) sementes nuas beneficiadas; 3) sementes pelotizadas; 4) sementes pelotizadas e coloridas. Para avaliação da qualidade das sementes nessas diferentes etapas, foram realizados os seguintes testes: germinação, primeira contagem de germinação, índice de velocidade de germinação, teste de emergência e índice de velocidade de emergência. Posteriormente, as sementes foram armazenadas por seis e doze meses e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nicotiana tabacum L.; Germinação; Emergência.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200216 |
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