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Registros recuperados: 13
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Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation Scientia Agricola
D'Agostini,Érica Clarissa; Mantovani,Talita Rafaele D'Agostini; Valle,Juliana Silveira do; Paccola-Meirelles,Luzia Doretto; Colauto,Nelson Barros; Linde,Giani Andrea.
Basidiomycetes are laccase producers used for hydrolysis of lignocellulosic byproducts in fermentative processes and could be used on biofuel production or ruminant feeding. The objective of this study was to evaluate the effect of concentrations of non-protein nitrogen sources on laccase production and mycelial growth of Pleurotus ostreatus, Lentinula edodes and Agaricus blazei. The fungi were grown on soybean hulls to which urea (U), ammonium sulfate (AS) or mixture of AS:U (1:1) were added to achieve carbon/nitrogen (C/N) ratios of 5, 15, 20 or 30. The average longitudinal mycelial growth was measured and laccase activity was determined by the oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Higher C/N ratios increased mycelial growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Industrial byproduct; Lignocellulolytic; Enzyme; Lignolytic; Soybean hull.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300004
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Novel insights into the early stages of infection by oval conidia of Colletotrichum sublineolum Scientia Agricola
Souza-Paccola,Ednéia Aparecida de; Bomfeti,Cleide Aparecida; Tanaka,Francisco André Ossamu; Massola Junior,Nelson Sidnei; Colauto,Nelson Barros; Figueiredo,José Edson Fontes; Paccola-Meirelles,Luzia Doretto.
Anthracnose, caused by Colletotrichum sublineolum Henn. ex Sacc. & Trotter, is one of the most important sorghum [Sorghum bicolor (L.) Moench] diseases in Brazil. This fungus exhibits conidial dimorphism, producing either falcate or oval conidia on solid and liquid media, respectively. We compared patterns of the initial infection events by these two types of conidia on sorghum leaves using light microscopy and scanning electron microscopy. The infection events during the first 24 h were similar for both oval and falcate conidia. Globose and melanized apressoria were formed at 24 h after inoculation (hai) regardless of the conidia type. Dense mycelium and oval conidia developed from germinated falcate conidia at 32 hai. Hyphal mass displaying acervuli...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorghum bicolor; Anthracnose; Conidial dimorphism; Infection biology; Secondary sporulation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000400351
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Antifungal activity of Gallesia integrifolia fruit essential oil BJM
Raimundo,Keila Fernanda; Bortolucci,Wanessa de Campos; Glamočlija,Jasmina; Soković,Marina; Gonçalves,José Eduardo; Linde,Giani Andrea; Colauto,Nelson Barros; Gazim,Zilda Cristiani.
ABSTRACT Gallesia integrifolia (Phytolaccaceae) is native to Brazil and has a strong alliaceous odor. The objective of this study was to identify the chemical composition of G. integrifolia fruit essential oil and evaluate fungicidal activity against the main food-borne diseases and food spoilage fungi. The essential oil was extracted by hydrodistillation and identified by GC–MS. From 35 identified compounds, 68% belonged to the organosulfur class. The major compounds were dimethyl trisulfide (15.49%), 2,8-dithianonane (52.63%) and lenthionine (14.69%). The utilized fungi were Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pau d’alho 2; 8-Dithianonane Lenthionine Aspergillus sp. Penicillium sp..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500229
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Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose BJM
Zaghi Júnior,Lienine Luiz; Lopes,Ana Daniela; Cordeiro,Fábio Aparecido; Colla,Itaruã Machri; Bertéli,Míria Benetati Delgado; Valle,Juliana Silveira do; Linde,Giani Andrea; Colauto,Nelson Barros.
Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20 °C is not effective to keep...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preservation; Freeze; Cereal grain; Monosaccharide; Agaricus blazei.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370
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Antioxidant activity of Agaricus brasiliensis basidiocarps on different maturation phases BJM
Mourão,Francielly; Umeo,Suzana Harue; Takemura,Orlando Seiko; Linde,Giani Andrea; Colauto,Nelson Barros.
Different maturation phases of basidiocarp could affect the bioactivity and concentration of some active substances. A. brasiliensis Wasser et al.(A. blazei Murrill) has shown antitumor activity that could be related to the antioxidant activity. However there is no information of the best basidiocarp maturation phase for extracting antioxidant substances in order to determine the moment of harvesting in mushroom cultivation. The objective of this work was to evaluate the antioxidant activity of A. brasiliensis strains on different basidiocarp maturation phases. The best condition for extraction of A. brasiliensis antioxidants is with methanol as solvent at 60 ºC for 60 min. Strains with closed basidiocarp have higher antioxidant activity than with opened...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Absorbance; Antioxidant activity; Basidiomycete; Agaricus blazei; DPPH.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100024
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Column bioreactor use for optimization of pectinase production in solid substrate cultivation BJM
Linde,Giani Andrea; Magagnin,Glênio; Costa,Jorge Alberto Vieira; Bertolin,Telma Elita; Colauto,Nelson Barros.
This study aimed to determine the influence of process variables and production, of polygalacturonase (PG) and polymetylgalacturonase (PMG) by solid substrate cultivation using a fixed bed column bioreactor. A fractional factorial design (FFD) was used to study the effect of the following variables: microorganism (Aspergillus oryzae and Aspergillus niger), substratum (wheat bran and defatted rice bran), aeration (40 and 60 ml h-1g-1), pectin (5 and 10 g g-1) and nitrogen (urea and ammonium sulfate). Microorganism, aeration and initial pectin were identified in FFD as significant variables (p<0.05) on PG production. A central composed design to optimize PG and PMG productions indicated that Aspergillus niger presents higher PG production; substratum and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid state fermentation; Agro-industrial residues; Pectinase.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300033
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Long-term cryopreservation of basidiomycetes BJM
Linde,Giani Andrea; Luciani,Alana; Lopes,Ana Daniela; Valle,Juliana Silveira do; Colauto,Nelson Barros.
Abstract Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation techniques is necessary once there is no universal protocol for the cryopreservation of basidiomycetes. Cryopreservation is a technique in which microorganisms are submitted to ultralow temperatures. Therefore, this study aimed to collect information on the main conditions for long-term cryopreservation of basidiomycetes in the last 20 years. Scientific articles on cryopreservation of basidiomycetes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preservation; Substrate; Fungi; Mycelial viability; Cryoprotectant.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200220
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Production flush of Agaricus blazei on Brazilian casing layers BJM
Colauto,Nelson Barros; Silveira,Adriano Reis da; Eira,Augusto Ferreira da; Linde,Giani Andrea.
This study aimed to verify the biological efficiency and production flushes of Agaricus blazei strains on different casing layers during 90 cultivation days. Four casing layers were used: mixture of subsoil and charcoal (VCS), lime schist (LSC), São Paulo peat (SPP) and Santa Catarina peat (SCP); and two genetically distant A. blazei strains. The fungus was grown in composted substratum and, after total colonization, a pasteurized casing layer was added over the substratum, and fructification was induced. Mushrooms were picked up daily when the basidiocarp veil was stretched, but before the lamella were exposed. The biological efficiency (BE) was determined by the fresh basidiocarp mass divided by the substratum dry mass, expressed in percentage. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Production flush; Agaricus brasiliensis; Casing layer; Water management; Agaricus subrufescens.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200026
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Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD BJM
Colauto,Nelson Barros; Dias,Eustáquio Souza; Gimenes,Marcos Aparecido; Eira,Augusto Ferreira da.
The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus blazei; Basidiomycete; Genetic diversity; Mushroom; RAPD.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200006
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Chemical characterization of Lippia alba essential oil: an alternative to control green molds BJM
Glamočlija,Jasmina; Soković,Marina; Tešević,Vele; Linde,Giani Andrea; Colauto,Nelson Barros.
The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by ¹H and 13C-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Essential oil; Neral; Geranial; Lippia alba.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400041
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Antineoplasic activity of Agaricus brasiliensis basidiocarps on different maturation phases BJM
Mourão,Francielly; Linde,Giani Andrea; Messa,Valdeci; Cunha Júnior,Paulo Luiz da; Silva,Aristeu Vieira da; Eira,Augusto Ferreira da; Colauto,Nelson Barros.
The fungus Agaricus brasiliensis is a Basidiomycete studied because of its immunomodulation and/or antitumor substances. The objective of this study was to verify the Agaricus brasiliensis antineoplasic activity in vivo on different basidiocarp maturation phases on Sarcoma 180 cells implanted in mice. Sarcoma cells were implanted in mice and after seven days mice were divided in three groups. The first group was treated with saline solution, the second group was treated with closed basidiocarp extract solution and the third group was treated with opened basidiocarp extract solution. After 30 days of being daily orally treated with these three solutions all animals suffered euthanasia, and the splenic index, tumor mass and volume were determined. No...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus blazei; Basidiome; Active principle; Antitumor; Fungus extract.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400022
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Regeneração de plantas a partir de dois tipos de explantes de milho submetidos à transformação genética por biobalística Ciência Rural
Vidigal,Tiago Mateus Andrade; Schuster,Ivan; Texeira,Leandra Regina; Colauto,Nelson Barros.
O uso da biobalística na transformação genética de plantas requer a otimização de diversos parâmetros, entre eles, o desenvolvimento de protocolos para regeneração de plantas a partir de células transformadas. Este trabalho teve como objetivo avaliar a eficiência de regeneração de plantas de milho a partir de calos embriogênicos e de explantes sementes divididas, submetidos à transformação por biobalística, utilizando o gene bar como marcador de seleção. Foram submetidos 2000 agrupamentos de calos embriogênicos de milho e 2000 explantes sementes divididas à transformação por biobalística. A seleção dos eventos de transformação foi realizada em meios de cultivo suplementados com glifosinato de amônio. As plântulas selecionadas foram aclimatadas e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milho; Embriogênese somática; Sementes divididas; Transformação genética; Gene bar..
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001804
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Tratamentos térmicos do calxisto para uso como camada de cobertura no cultivo de Agaricus brasiliensis Ciência Rural
Colauto,Nelson Barros; Silveira,Adriano Reis da; Eira,Augusto Ferreira da; Linde,Giani Andrea.
A escolha da camada de cobertura é uma das mais importantes etapas do cultivo de Agaricus brasiliensis. Apesar dessa importância, poucos estudos relatam o uso de diferentes tratamentos térmicos para o controle da microbiota em camadas de cobertura alternativas. Assim, o objetivo deste trabalho foi avaliar o efeito da pasteurização e da autoclavagem do material alternativo calxisto para utilização como camada de cobertura no cultivo de A. brasiliensis. O fungo foi inicialmente crescido em grãos de trigo e transferido para meio de cultivo previamente compostado. Após a completa colonização, a camada de cobertura (calxisto) pasteurizada ou autoclavada foi adicionada. Avaliaram-se a eficiência biológica, o número e a biomassa de cogumelos produzidos e o fluxo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eficiência biológica; Agaricus blazei; Massa do cogumelo; Tratamento térmico.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000700028
Registros recuperados: 13
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