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Registros recuperados: 5
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Long-term evaluation of the antimicrobial susceptibility and microbial profile of subgingival biofilms in individuals with aggressive periodontitis BJM
Lourenço,Talita Gomes Baêta; Heller,Débora; Souto,Renata Martins do; Silva-Senem,Mayra Xavier e; Varela,Victor Macedo; Torres,Maria Cynesia Barros; Feres-Filho,Eduardo Jorge; Colombo,Ana Paula Vieira.
This study evaluates the antimicrobial susceptibility and composition of subgingival biofilms in generalized aggressive periodontitis (GAP) patients treated using mechanical/antimicrobial therapies, including chlorhexidine (CHX), amoxicillin (AMX) and metronidazole (MET). GAP patients allocated to the placebo (C, n = 15) or test group (T, n = 16) received full-mouth disinfection with CHX, scaling and root planning, and systemic AMX (500 mg)/MET (250 mg) or placebos. Subgingival plaque samples were obtained at baseline, 3, 6, 9 and 12 months post-therapy from 3–4 periodontal pockets, and the samples were pooled and cultivated under anaerobic conditions. The minimum inhibitory concentrations (MICs) of AMX, MET and CHX were assessed using the microdilution...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aggressive periodontitis; Biofilms; Microbial sensitivity tests; DNA probes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200493
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Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis BJM
Rosalem Junior,Wilson; Souza,Rodrigo Carvalho de; Andrade,Arnaldo Feitosa Braga de; Colombo,Ana Paula Vieira.
Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Generalized aggressive periodontitis; PCR; Actinobacillus actinomycetemcomitans; Saliva; Leukotoxin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006
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Prevalence of leukotoxic genotypes of Actinobacillus actinomycetemcomitans in Brazilians with chronic periodontitis BJM
Rosalem Junior,Wilson; Andrade,Arnaldo Feitosa Braga de; Colombo,Ana Paula Vieira.
Actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. This investigation determined the prevalence of A. actinomycetemcomitans and the occurrence of the leukotoxin gene 530-bp deletion in Brazilian subjects with chronic periodontitis. Twenty periodontally healthy and 20 chronic periodontitis subjects were selected. Full-mouth clinical examination was carried out at 6 sites/tooth. Subgingival biofilm samples were collected from the 3 deepest sites and 3 healthy sites from periodontitis subjects, as well as from 3 sites of individuals with periodontal health....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronic periodontitis; PCR; Actinobacillus actinomycetemcomitans; Subgingival biofilm; Leukotoxin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400036
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Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection BJM
Souto,Renata; Silva-Boghossian,Carina M.; Colombo,Ana Paula Vieira.
P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Acinetobacter spp; Subgingival biofilm; Saliva; Periodontitis; PCR.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200017
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Prevalence of "non-oral" pathogenic bacteria in subgingival biofilm of subjects with chronic periodontitis BJM
Souto,Renata; Andrade,Arnaldo Feitosa B. de; Uzeda,Milton; Colombo,Ana Paula Vieira.
The oral cavity may act as a reservoir for several pathogens related to systemic infections. Therefore, the purpose of this study was to determine the prevalence and levels of pathogenic bacteria in the subgingival biofilm of chronic periodontitis lesions and healthy periodontal sites using the checkerboard DNA-DNA hybridization technique. 200 samples of subgingival biofilm from sites with periodontitis (probing pocket depth > 4 mm and /or clinical attachment level > 4 mm) and 200 samples from healthy sites of 14 patients with chronic periodontitis, as well as 200 samples from 3 periodontally healthy patients were obtained. The presence and levels of 11 pathogenic bacteria were determined using whole genomic DNA probes and the checkerboard method,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: "non-oral" bacteria; Periodontal disease; Checkerboard; DNA probes.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300002
Registros recuperados: 5
Primeira ... 1 ... Última
 

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