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| Registros recuperados: 63 | |
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| Colas, Florent; Laurent, Sebastien; Lehaitre, Michel; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Dreanno, Catherine; Deuff, Albert; Compere, Chantal. |
| For the last few years there is a more and more pressing need to develop system for detecting HAB at their early stage. Researchers have then been working on new in situ sensor. In this paper a submarine transducer based on surface plasmon resonance is reported. It demonstrated a refractive index resolution of 3.10(-6), which is promising for later phytoplankton detection. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00107/21802/20328.pdf |
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| Anne, Marie-laure; Keirsse, Julie; Nazabal, Virginie; Hyodo, Koji; Inoue, Satoru; Boussard-pledel, Catherine; Lhermite, Herve; Charrier, Joel; Yanakata, Kiyoyuki; Loreal, Olivier; Le Person, Jenny; Colas, Florent; Compere, Chantal; Bureau, Bruno. |
| Due to the remarkable properties of chalcogenide (Chg) glasses, Chg optical waveguides should play a significant role in the development of optical biosensors. This paper describes the fabrication and properties of chalcogenide fibres and planar waveguides. Using optical fibre transparent in the mid-infrared spectral range we have developed a biosensor that can collect information on whole metabolism alterations, rapidly and in situ. Thanks to this sensor it is possible to collect infrared spectra by remote spectroscopy, by simple contact with the sample. In this way, we tried to determine spectral modifications due, on the one hand, to cerebral metabolism alterations caused by a transient focal ischemia in the rat brain and, in the other hand, starvation... |
| Tipo: Text |
Palavras-chave: Planar waveguide; Fibre; Optical sensor; Chalcogenide. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6851.pdf |
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| Quiniou, Francoise; Compere, Chantal; Caisey, Xavier; Davy, Romain; Matarere, Marcel; Mazeas, Florence; Peleau, Michel. |
| Since 1998, the European Biocidal Products Directive (BPD; 98/8/EC), applied to 23 product types (PT) for specific uses, including PT 21 the « antifouling products », regulates biocidal products before they are placed on the market. On the one hand, the efficacy of active substances and their formulations must be demonstrated for their specific employment ; on the other hand their non impact on workers neither users and environment must be proved and specifically against non target species. Standard protocols for the assessment of efficacy or ecotoxicity of antifouling substances are proposed within the framework of the Ecopaint and Paintclean projects which are founded by the french industry Ministery and labellised by the “Pôle Mer” (Paca and... |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00040/15133/12471.pdf |
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| Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain. |
| Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms. |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf |
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| Delauney, Laurent; Compere, Chantal; Lehaitre, Michel. |
| These days, many marine autonomous environment monitoring networks are set up in the world. These systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or underwater oceanographic structures. These systems commonly use various sensors to measure parameters such as dissolved oxygen, turbidity, conductivity, pH or fluorescence. Emphasis has to be put on the long term quality of measurements, yet sensors may face very short-term biofouling effects. Biofouling can disrupt the quality of the measurements, sometimes in less than a week. Many techniques to prevent biofouling on instrumentation are listed and studied by researchers and manufacturers. Very few of them... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00008/11920/8626.pdf |
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| Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie. |
| Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal... |
| Tipo: Text |
Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf |
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| Dheilly, Alexandra; Soum-soutera, Emmanuelle; Klein, Geraldine; Bazire, Alexis; Compere, Chantal; Haras, Dominique; Dufour, Aurelie. |
| Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and... |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00004/11530/11317.pdf |
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| Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal. |
| Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate. |
| Tipo: Text |
Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf |
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| Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie. |
| Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved. |
| Tipo: Text |
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf |
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| Klein, Geraldine; Dufour, Alain; Compere, Chantal. |
| Lors d’une immersion en eau de mer, tous les matériaux et structures sont rapidement colonisés par des salissures d’origine biologique : des biofilms. La transformation des propriétés de surface dumatériau et la présence demicro- oumacro-organismes (bactéries, algues, balanes, larves) engendrent des risques accrus de corrosion localisée, la biodétérioration desmatériaux immergés, le blocage des fonctions mécaniques…Afin de contrôler le dépôt et le développement de ces biofilms, la méthode privilégiée est l’application de peintures anti-salissures. Cependant ces revêtements sont généralement toxiques pour l’environnement. C’est pourquoi, les travaux menés actuellement visent à isoler de nouvelles molécules, produites par les bactéries marines, dans le but... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00351/46213/45910.pdf |
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| Dridi, Montacer; Colas, Florent; Compere, Chantal. |
| For the last few years, plasmon-based lasers have been investigated theoretically and experimentally. Several configurations have been reported. They are composed of quantum emitters coupled to a plasmonic structure. In this paper, we investigate the effect of the plasmon mode on the far-field and near-field characteristics of a nanolaser composed by a periodic array of metallic nanoparticles covered by gain materials under optical pumping. Two configurations were investigated. The first structure supported a localized surface plasmon (LSP) mode, while the second a surface lattice plasmon (SLP) mode. This theoretical work relies on semi-quantum calculations based on a four-level gain molecule and a time-domain approach. We demonstrated that lasing in a SLP... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00473/58490/61054.pdf |
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| Garcia-martinez, Gonzalo; Alonso Bustabad, Enrique; Perrot, Hubert; Gabrielli, Claude; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodriguez-pardo, Loreto; Farina, Jose; Compere, Chantal; Arnau Vives, Antonio. |
| This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected. |
| Tipo: Text |
Palavras-chave: DNA sequence detection; Quartz crystal microbalance; Electronic oscillator; Sensitivity; Frequency noise; Resolution. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00044/15487/12886.pdf |
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| Compere, Chantal. |
| Introduction: Almost all submerged surfaces in the marine environment are subject to developing an organic film, with the exception of some living organisms, such as sponges, which do not undergo any great colonization, for no currently-known scientific reason. The presence of this biofilm is at the source of many problems in the oceanographic field and for marine business. This article introduces some drawbacks relating to the existence of this fouling and elaborates upon the main studies conducted at IFREMER concerning the most-used methods for combating it. However, the anti-fouling agents that are generally used for protecting facilities are toxic and can have disastrous consequences on the marine environment's fauna and flora. Moreover, the use of... |
| Tipo: Text |
Palavras-chave: Biofilm; Seawater. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-1698.pdf |
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| Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.. |
| The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and... |
| Tipo: Text |
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf |
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| Verger, F.; Pain, T.; Nazabal, V.; Boussard-pledel, C.; Bureau, Bruno; Colas, Florent; Rinnert, Emmanuel; Boukerma, Kada; Compere, Chantal; Guilloux-viry, M.; Deputier, S.; Perrin, A.; Guin, J. P.. |
| The development of infrared sensing platform requires adapted materials. In this work, we used sputtered gold nanoparticles on a chalcogenide glass to demonstrate that these infrared materials can be used as substrate for surface enhanced infrared absorption (SEIRA) spectroscopy. The sputtering parameters were optimized to get the highest possible enhancement. To assess it, a self-assembled monolayer of 4-nitrothiophenol was used. These preliminary results are promising and pave the way for various configurations of waveguide for integrated optical components. (C) 2012 Elsevier B.V. All rights reserved. |
| Tipo: Text |
Palavras-chave: Chalcogenide glass; Sputtering; Gold nanoparticles; 4-Nitrothiophenol; SEIRA; Surface enhanced infrared absorption. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00116/22748/20613.pdf |
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| Registros recuperados: 63 | |
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