|
|
|
|
|
| Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia. |
| Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629 |
| |
|
|
| Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa. |
| Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086 |
| |
|
| |
|
|
| Coelho,Mônica Bossardi; Scagliusi,Sandra Maria Mansur; Lima,Maria Imaculada Pontes Moreira; Consoli,Luciano; Grando,Magali Ferrari. |
| Abstract: The objective of this work was to assess the androgenic response, via microspore culture, of wheat genotypes with different levels of resistance to Gibberella zeae. The number of androgenic embryos per spike, and of green and albino plants was counted for the BRS 179 (moderately resistant), Frontana and Sumai 3 (resistant), and BRS 194, Embrapa 27, and Fielder (susceptible) genotypes. The degree of interference by the Fielder, Pavon 76, and Sumai 3 ovary-donor genotypes, used for co-culture with the microspore cells, was also assessed regarding androgenic response. Induction efficiency ranged from 0.33 embryo per spike for Embrapa 27 to 109.8 embryos for Frontana. Sumai 3 presented the second best response, and Embrapa 27 behaved as highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gibberella zeae; Triticum aestivum; Doubled haploids; Microspore. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500575 |
| |
|
| |
|
|
| Lefèvre,Agnès; Consoli,Luciano; Gaziola,Salete A.; Pellegrino,Ana Paula; Azevedo,Ricardo A.; Damerval,Catherine. |
| The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The structural polymorphism at the O2 locus was investigated by RFLP in a collection of 51 maize inbred lines. Most polymorphic sites were found outside the coding regions. We then searched for relationships between RFLP polymorphism and (i) mRNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zein; Aspartate kinase; Lysine oxoglutarate reductase/saccharopine dehydrogenase; Polymorphism. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200031 |
| |
|
|
|
