SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Cazetta,Marcia Luciana; Monti,Rubens; Contiero,Jonas.

The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.

Purification of papain from fresh latex of Carica papaya

Palavras-chave: Kluyveromyces marxianus var. bulgaricus; Sucrose; Inulinase; Biomass; Batch fermentation.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027

Monti,Rubens; Basilio,Carmelita A.; Trevisan,Henrique C.; Contiero,Jonas.

In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.

Microbial rennet produced by Mucor miehei in solid-state and submerged fermentation

Palavras-chave: Papain; Carica papaya; Cysteine proteinase; Proteolytic enzymes; Purification of papain.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500009

Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas.

The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively....

Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils

Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009

Mariano,Adriano Pinto; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas; Bonotto,Daniel Marcos.

This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation experiments were...

Isolation of natural inhibitors of papain obtained from Carica papaya latex

Palavras-chave: Bioremediation; Bioaugmentation; Soil; Diesel oil; Service station.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500030

Monti,Rubens; Contiero,Jonas; Goulart,Antonio José.

Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).

L-(+)-Lactic acid production by Lactobacillus rhamnosus B103 from dairy industry waste

Palavras-chave: Esterase activity; Natural papain inhibitor; Protease inhibitor.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010

Bernardo,Marcela Piassi; Coelho,Luciana Fontes; Sass,Daiane Cristina; Contiero,Jonas.

ABSTRACT Lactic acid, which can be obtained through fermentation, is an interesting compound because it can be utilized in different fields, such as in the food, pharmaceutical and chemical industries as a bio-based molecule for bio-refinery. In addition, lactic acid has recently gained more interest due to the possibility of manufacturing poly(lactic acid), a green polymer that can replace petroleum-derived plastics and be applied in medicine for the regeneration of tissues and in sutures, repairs and implants. One of the great advantages of fermentation is the possibility of using agribusiness wastes to obtain optically pure lactic acid. The conventional batch process of fermentation has some disadvantages such as inhibition by the substrate or the final...

Cyclodextrin glycosyltransferase production by new Bacillus sp. strains isolated from brazilian soil

Palavras-chave: Feed strategies; Lactic acid production; Lactobacillus and lactic acid purification.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300640

Menocci,Vivian; Goulart,Antonio José; Adalberto,Paulo Roberto; Tavano,Olga Luisa; Marques,Daniela Parreira; Contiero,Jonas; Monti,Rubens.

Three strains of Bacillus sp. (BACRP, BACNC-1 and BACAR) were isolated from soil adhered to cassava husk. CGTase specific activity for the three isolated strains was higher when cultivated at 40ºC. Potato starch, cassava starch, maltodextrin and glucose were used as carbon source and growth temperatures varied from 25 to 55ºC. The three isolates presented higher CGTase specific activity when cultivated with potato starch at 40ºC. Isolated BACRP and BACAR presented specific activity of 4.0x10-3 and 2.2x10-3 U/mg prot at pH 7.0, respectively, when cultivated in mediums added with NaCl 2%; at pH 10,0 their activities were of 3.4x10-3 and 3.0x10-3 U/mg prot, respectively, in the same concentration of NaCl. On the other hand, the isolated BACNC-1 presented...

Biodegradability of commercial and weathered diesel oils

Palavras-chave: Cyclodextrin glycosyltransferase; CGTase production.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400016

Mariano,Adriano Pinto; Bonotto,Daniel Marcos; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas.

This work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. Two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 - dichlorophenol indophenol (DCPIP) and soil respirometric experiments using biometer flasks. In the former we tested the bacterial cultures Staphylococcus hominis, Kocuria palustris, Pseudomonas aeruginosa LBI, Ochrobactrum anthropi and Bacillus cereus, a commercial inoculum, consortia obtained from soil and groundwater contaminated with hydrocarbons and a consortium from an uncontaminated area. In the respirometric experiments it was...

Production and purification of an Endo1,4-beta-Xylanase from Humicola grisea var. thermoidea by electroelution

Palavras-chave: Biodegradability; Bioremediation; Commercial oil diesel; Weathered diesel oil.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100028

Monti,Rubens; Cardello,Leonardo; Custódio,Marcos F.; Goulart,Antonio J.; Sayama,Adriana H.; Contiero,Jonas.

Humicola grisea var. thermoidea produces two forms of extracellular xylanase. The component form 1 was purified using the electroelution method, due to the very small production of this extracellular enzyme. The apparent molecular mass was 61.8 kDa by SDS-PAGE.

Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry

Palavras-chave: Electroelution; Enzyme purification; Extracellular xylanase; Humicola grisea var. thermoidea.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200007

Freitas,Telma Luisa de; Monti,Rubens; Contiero,Jonas.

GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.

Produção de biotensoativos a partir de resíduos de óleos e gorduras

Palavras-chave: Bacillus alkalophilic CGII; CGTase; Beta-cyclodextrin; Starch hydrolysis; Enzyme activity.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015

Ciênc. Tecnol. Aliment.

Costa,Siddhartha Georges Valadares Almeida de Oliveira; Nitschke,Márcia; Contiero,Jonas.

O presente trabalho visou a seleção de microrganismos com capacidade de produzir biotensoativos a partir de resíduos de óleos e gorduras gerados em restaurantes e indústrias alimentícias. Borra de soja, gordura de frango, gordura vegetal hidrogenada e óleo de soja usado em frituras foram estudados como fonte de carbono. Os isolados LMI 6c e LMI 7a, ambos pertencentes ao gênero Pseudomonas, foram selecionados como potenciais produtores de biotensoativos. Dentre os resíduos propostos, a borra de soja foi considerada o melhor substrato, gerando 9,69 g.L-1 de ramnolipídios e uma tensão superficial de 31 mN/m.