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Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program Scientia Agricola
Zanutto,Carlos Alexandre; Corazza,Maria Júlia; Nunes,William Mário de Carvalho; Müller,Gerd Walter.
The use of tolerant rootstocks and preimmunization has satisfactorily controlled losses associated with the Citrus tristeza virus (CTV). Several researchers have shown that CTV mild isolates that are selected in the same region where they are used are superior to isolates obtained from other areas. Thus, budwoods of 20 outstanding citrus trees were collected in north and northwestern Paraná state (Brazil) citrus-producing areas and established to be used in a preimmunization program. These budwoods were tested to evaluate the potential protection of the inherently present viral complex. Based on biological indexing and molecular characterization of the capsid protein gene by RFLP (restriction fragment length polymorphism, which indicated that the plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SSCP; RFLP; Cross-protection; Citrus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200009
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