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Mathias,Luis Antonio; Corbellini,Luis Gustavo; Maia,Lúcia; Nascimento,Kelly Fagundes; Paulin,Lília Márcia Silva; Samartino,Luis Ernesto; Serqueira,Marco Antonio; Soares Filho,Paulo Martins; Souza,Marcília Maria Alves de. |
Esta investigação teve por objetivo validar o teste de polarização fluorescente (TPF) para o diagnóstico sorológico da brucelose bovina, determinando a sensibilidade (SE) e a especificidade relativas (SP) e verificando a reprodutibilidade do teste em quatro laboratórios no Brasil. Foram selecionadas 1.389 amostras de soro sanguíneo, as quais foram inicialmente submetidas aos testes do antígeno acidificado tamponado (AAT) e mercaptoetanol (2-ME). As mesmas amostras foram submetidas à reação de fixação de complemento (RFC) e ao TPF. Para a avaliação do TPF, foi adotada a combinação dos resultados do AAT, da RFC e do 2-ME, utilizados como população de referência (padrão-ouro). Para a determinação do ponto de corte do TPF que proporciona a melhor combinação de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucelose bovina; Diagnóstico sorológico; Teste de polarização fluorescente. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000013 |
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Pescador,Caroline Argenta; Corbellini,Luis Gustavo; Driemeier,David; Gonçalves,Ricardo Kalil; Cruz,Cláudio Estêvão Farias. |
A two-month-old lamb showing signs of severe neurological disease characterized by muscular tremors, hypermetria, and motor incoordination was submitted to the Veterinary Pathology Laboratory - Universidade Federal do Rio Grande do Sul, Brazil. At necropsy, the major findings were a marked reduction of the size of the cerebellum and bilateral dilatation of the lateral ventricles. Microscopically, areas of cellular disorganization in the cerebellar cortex, reduction of the granular layer of cerebellum associated with decreased density of cells, and the presence of large cytoplasmic vacuoles in the molecular layer were observed. Neurons of the gray matter of the brain and macrophages of the mesenteric lymph nodes stained positively by the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pestivirus; Border Disease; Sheep; Brazil. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300044 |
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Lunkes,Vinícius Leobet; Tonin,Alexandre Alberto; Machado,Gustavo; Corbellini,Luis Gustavo; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Bezerra,Camila de Sousa; Azevedo,Sérgio Santos de; Pequeno,Nebson Fernandes; Silva,Adriana Moraes da; Weiblen,Rudi; Flores,Eduardo Furtado. |
ABSTRACT: Vesicular stomatitis virus (VSV) is the agent of a vesicular disease that affects many animal species and may be clinically confounded with foot-and-mouth disease in ruminant and swine. Horses are especially susceptible to VSV and may serve as sentinels for virus circulation. The present study investigated the presence of neutralizing antibodies against VSV Indiana III (VSIV-3) in serum samples of 3,626 horses from six states in three Brazilian regions: Southern (RS, n = 1,011), Midwest (GO/DF, n = 1,767) and Northeast (PB, PE, RN and CE, n = 848) collected between 2013 and 2014. Neutralizing antibodies against VSIV-3 (titers ≥40) were detected in 641 samples (positivity of 17.7%; CI95%:16.5-19.0%), being 317 samples from CE (87.3%; CI95%:... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serology; Differential diagnosis; Foot-and-mouth disease; Zoonosis; Vesicular disease. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801424 |
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Weiblen,Carla; Machado,Gustavo; Jesus,Francielli Pantella Kunz de; Santurio,Janio Morais; Zanette,Régis Adriel; Pereira,Daniela Sabel Brayer; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Corbellini,Luis Gustavo; Botton,Sônia de Avila. |
ABSTRACT: An epidemiological survey was carried out by performing an Enzyme Linked Immuno Sorbent Assay (ELISA) test to determine the seroprevalence of Pythium insidiosum infection in equine in Rio Grande do Sul State (RS), Brazil. The serological study covered seven geographical regions of RS, classified according to the Instituto Brasileiro de Geografia e Estatística (IBGE). The samples were obtained from official veterinary service (Serviço Veterinário Oficial, SVO) linked to the Secretaria da Agricultura, Pecuária e Agronegócio of RS (SEAPA-RS) to proceed the investigation of equine infectious anemia in 2014. Samples were collected during the months of September and October of 2013, covering the seven geographical regions of RS, and totalized 1,002... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: P. insidiosum; Antibody; Occurrence; Horses; ELISA. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100126 |
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