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	Provedor de dados BABT (1) Rev. Microbiol. (1) Autor Correia,Marcos José (2) Cavalcanti,Maria Auxiliadora de Queiroz (1) Oliveira,Neiva Tinti de (1) Pereira Junior,José Antônio de Sousa (1) Pereira Júnior,José Antônio de Sousa (1) Santos,Jefferson Cunha dos (1) Mais... Palavra-chave Cellulase (2) Avicel (1) Lentinula edodes (1) Lentinus edodes (1) Liquid medium cultivation (1) Pleurotus ostreatus (1) Remazol Blue (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 1998 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes Rev. Microbiol. Correia,Marcos José; Pereira Junior,José Antônio de Sousa; Santos,Jefferson Cunha dos; Cavalcanti,Maria Auxiliadora de Queiroz. A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm). Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicel; Cellulase; Remazol Blue; Lentinus edodes; Pleurotus ostreatus. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010 Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose BABT Pereira Júnior,José Antônio de Sousa; Correia,Marcos José; Oliveira,Neiva Tinti de. Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lentinula edodes; Cellulase; Liquid medium cultivation. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300004 Registros recuperados: 2 Primeira ... 1 ... Última

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