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	Provedor de dados 56 (1) 63 (1) Autor Corrente,J.E. (2) Alvares,L.E. (1) Coutinho,L.L. (1) Kiehl,J.C. (1) Mantoani,A. (1) Pismel,F.S. (1) Prochnow,L.I. (1) Mais... Palavra-chave Ammonia losses (1) Gene expression (1) Manure composting (1) Myogenic regulatory factors (1) Phosphogypsum (1) Quantitative RT-PCR (1) Simple superphosphate (1) Standard-curve (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 1995 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos 56 Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L.. The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004 Controlling ammonia losses during manure composting with the addition of phosphogypsum and simple superphosphate 63 Prochnow,L.I.; Kiehl,J.C.; Pismel,F.S.; Corrente,J.E.. With the purpose of evaluating the effectiveness of phosphogypsum and simple superphosphate in controlling ammonia losses by volatilization during the process of manure composting, an experiment was carried out in closed flasks of 1.6 liter, in which different rates (50, 100, 150 and 200 kg.t-1) of phosphogypsum and simple superphosphate were mixed with a substrate composed of equal amounts (on weight basis) of fresh chicken manure and cattle manure. Ammonia lost by volatilization was determined after 7, 14, 21, 28 and 35 days by trapping the gas in sulfuric acid solution placed in a small glass vial which, in turn, was maintained inside the volatilization flask The remaining acid after the exposure to ammonia was determined by titration with 0.025N NaOH.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ammonia losses; Phosphogypsum; Simple superphosphate; Manure composting. Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000200024 Registros recuperados: 2 Primeira ... 1 ... Última

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