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| Alves,Luciane Martins; Soares,Rodrigo Martins; Cortez,Adriana; Richtzenhain,Leonardo José; Ito,Fumio Nonma. |
| Hamsters orally inoculated with ERA and PV strains of rabies virus were sacrificed at 24, 48, 72 hours, 21 and 30 days after inoculation. Brain fragments were examined by Fluorescent Antibody test (FAT) and heminested PCR (hn-PCR). Fragments from stomach, blood, heart, and lung were examined only by hn-PCR. Sera of other hamsters, similarly inoculated, obtained at 30th day after inoculation were submitted to mouse neutralization test. The hamsters were challenged intracerebrally with CVS strain with 10(2.7)mouse LD50/0.03mL, 45 days after inoculation. Brains examined by FAT were negative. The hn-PCR detected the presence of rabies virus RNA in the lung of one animal inoculated with ERA, and in the brain, stomach, blood, and lung of PV-infected animals. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies; Oral administration; ERA and PV strains; Hn-PCR; Hamster. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000100010 |
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| Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio. |
| Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003 |
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| Heinemann,Marcos Bryan; Cortez,Adriana; Souza,Maria do Carmo Custódio de; Gotti,Tatiana; Ferreira,Fernando; Homem,Valéria Stachini Ferreira; Ferreira Neto,José Soares; Soares,Rodrigo Martins; Sakamoto,Sidnei Mioshi; Cunha,Elenice Maria Sequetin; Richtzenhain,Leonardo José. |
| Os vírus da anemia infecciosa eqüina (VAIE), da arterite viral dos eqüinos (VAVE) e do aborto viral eqüino (Herpesvírus eqüino tipo 1, HVE-1) são agentes causadores de enfermidades nos eqüídeos que podem causar graves prejuízos econômicos. O objetivo do presente trabalho foi estimar a soroprevalência de anticorpos contra os vírus VAIE, VAVE e HVE-1, utilizando como unidades de análise os eqüídeos e as propriedades rurais do tipo familiar do município de Uruará, PA. Os anticorpos contra o VAIE foram pesquisados pela prova de imunodifusão em gel de ágar e os anticorpos contra o VAVE e o HVE-1 pela prova de soroneutralização. O tamanho da amostra foi estimado a partir de um total de 2069 propriedades, caracterizadas por agricultura familiar e ausência de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prevalência; Uruará (PA); Anemia infecciosa eqüina; Arterite animal. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100009 |
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| Yai,Lucia Eiko Oishi; Vianna,Manoella Compostrini Barreto; Soares,Rodrigo Martins; Cortez,Adriana; Freire,Roberta Lemos; Richtznhain,Leonardo José; Gennari,Solange Maria. |
| The aim of the present experiment was to standardize a nested polymerase chain reaction (nPCR) for the detection of Toxoplasma gondii in tissues of experimentally infected pigs and to compare the performance of nPCR with the standard isolation technique, the bioassay in mice. Comparison between the two methods was done testing eight 4 month-old pigs orally inoculated with 5 x 10(4) oocysts of Toxoplasma gondii (AS-28 strain) and three non-infected pigs at the same age, kept as control. All animals were euthanatized 47 days after infection and samples of brain, heart, tongue and retina were collected from each animal for analysis by nPCR and bioassay in mice. By using the bioassay, Toxoplasma gondii was detected in 4 infected pigs, being two in the retina,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Pig; NestedPCR; Bioassay; Diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000300010 |
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| Piatti,Rosa Maria; Ikuno,Alice Akimi; Cunha,Elenice Sequetin; D'Ambros,Régia; Gregori,Fábio; Soares,Rodrigo Martins; Cortez,Adriana; Richtzenhain,Leonardo José. |
| The genomic DNA of thirty strains of Aujeszky's disease virus (ADV) isolated in the South and Southeast regions of Brazil from 1982 to 1996 were characterized by restriction endonuclease analysis with BamHI. Twenty seven strains were isolated from pigs, 1 from cattle, 1 from cat and 1 from dog. Using a systematization previously described, the 30 ADV strains could be classified as genomic types I (n = 2) and II (n = 28). Genomic type III was not observed. In this first study of genomic type characterization of brazilian ADV strains, we could demonstrate the occurence in Brazil of the genomic types I and II, with a large predominance of genomic type II. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aujeszky's disease virus; Swine; Genomic RFLP; Brazil. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200015 |
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| Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna; Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda. |
| Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospires; Semen; Antibiotics; Extender. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011 |
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| Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima. |
| Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028 |
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