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	Provedor de dados PAB (1) Autor Costa,Alessandra Tenório (1) Müller,Gerd Walter (1) Nunes,William Mário de Carvalho (1) Zanutto,Carlos Alexandre (1) Palavra-chave Citrus sinensis (1) Cross-protection (1) Preimmunization (1) Single strand conformational polymorphism (1) Stem pitting symptoms (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2010 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Stability of Citrus tristeza virus protective isolates in field conditions PAB Costa,Alessandra Tenório; Nunes,William Mário de Carvalho; Zanutto,Carlos Alexandre; Müller,Gerd Walter. The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Cross-protection; Preimmunization; Single strand conformational polymorphism; Stem pitting symptoms. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700009 Registros recuperados: 1 Primeira ... 1 ... Última

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