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Registros recuperados: 3
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Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao PAB
Silva,Thiago Édson Ribeiro da; Cidade,Luciana Cardoso; Alvim,Fátima Cerqueira; Cascardo,Júlio Cézar de Mattos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Cacao; Gas exchange; Micropropagation; Morphogenesis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000024
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Tissue culture parameters in sweet orange cultivars PAB
Mendes,Amanda Ferreira da Silva; Cidade,Luciana Cardoso; Manzoli,Gabrielle Novais; Otoni,Wagner Campos; Soares Filho,Walter dos Santos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020
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Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars PAB
Oliveira,Maria Luiza Peixoto de; Costa,Marcio Gilberto Cardoso; Silva,Crislene Viana da; Otoni,Wagner Campos.
The objective of this study was to evaluate the effects of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) combinations, basal media and beta-lactam antibiotics on in vitro organogenesis from mature stem segments of 'Pêra', 'Valência' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige and Skoog (MS) medium containing the following BAP/NAA concentrations: 0.0/0.0; 0.25/0.0; 0.25/0.25; 0.5/0.0; 0.5/0.5; 1.0/0.0; 2.0/0.0; 2.0/0.25; 2.0/0.5; and 2.0/1.0 mg L-1. In order to test the influence of the culture media on shoot-bud induction, (MS), Murashige and Tucker (MT), and woody plant medium (WPM) formulations were evaluated, associated with the best...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus limonia; C. sinensis; Adult tissues; Antibiotics; Organogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700004
Registros recuperados: 3
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