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Registros recuperados: 7
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Marteiliosis in mussels: a rare disease? ArchiMer
Gombac, Mitja; Kusar, D.; Ocepek, M.; Pogacnik, Milan; Arzul, Isabelle; Couraleau, Yann; Jenclc, V..
Among 1280 cultured and wild adult Mediterranean mussels, Mytilus galloprovincialis, collected over a 1-year surveillance period from the Slovene Adriatic Sea, 0.3% were histologically positive for the presence of Marteilia spp. The infection was concentrated in winter. Employing the molecular methods of PCR, cloning, DNA restriction and sequencing, only Marteilia refringens type M was detected in all the infected mussels. Although all life-cycle stages of M. refringens severely infected digestive glands, only sporadic disruption of epithelial cells of digestive tubules and focal destruction of digestive tubules were observed in the infected mussels. This was the first detection of M. refringens in M. galloprovincialis from the Slovene Adriatic Sea with...
Tipo: Text Palavras-chave: Prevalence; Marteilia refringens type M.; Mediterranean mussels Mytilus galloprovincialis; Sequencing; Severe infection; Slovene Adriatic Sea.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00165/27640/26064.pdf
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Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa ArchiMer
Prado-alvarez, Maria; Couraleau, Yann; Chollet, Bruno; Tourbiez, Delphine; Arzul, Isabelle.
Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where...
Tipo: Text Palavras-chave: Bonamia exitiosa; Bonamia ostreae; Actin; Haplosporidia; Whole-Genome Amplification.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00277/38871/37410.pdf
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Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia ArchiMer
Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf
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Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis ArchiMer
Prado Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle.
In this study we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis haemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae infected haemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in haemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions.
Tipo: Text Palavras-chave: Bivalve haemocytes; Gene; Haplosporidia; Pathogen; Protist; Radicicol.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24799/22878.pdf
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Ostreid herpesvirus 1 infection in farmed Pacific oyster larvae Crassostrea gigas (Thunberg) in Korea ArchiMer
Hwang, J. Y.; Park, J. J.; Yu, H. J.; Hur, Y. B.; Arzul, Isabelle; Couraleau, Yann; Park, M. A..
Tipo: Text Palavras-chave: Crassostrea gigas; Korea; Ostreid herpesvirus 1; Pacific oyster.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00162/27276/26231.pdf
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Can the protozoan parasite Bonamia ostreae infect larvae of flat oysters Ostrea edulis? ArchiMer
Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine.
Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled...
Tipo: Text Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf
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PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3 ArchiMer
Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf
Registros recuperados: 7
Primeira ... 1 ... Última
 

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