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Drain, Alice; Bonneau, Laurent; Recorbet, Ghislaine; van Tuinen, Diederik; Wipf, Daniel; Courty, Pierre-Emmanuel. |
We developed a new protocol to study arbuscular mycorrhizal fungal (AMF) communities in Bordeaux vineyards in a standardized way, in order to compare results obtained over years and between locations. To this end, we first used grapevine root samples instead of soil samples to avoid AMF spores or hyphae interacting with cover plants. We next increased the number of grapevine samples to obtain more representative coverage of AMF communities while decreasing variability intra-vineyard, especially for the larger parcels. In addition, we adapted the DNA extraction protocol dedicated to soil samples to grapevine roots, as a way to increase the yield and the purity of samples. These features, coupled to the choice of the LSU-D2 region as the molecular marker for... |
Tipo: Book chapter |
Palavras-chave: Crop combinations and interactions Biodiversity and ecosystem services Crop health; Quality; Protection Viticulture. |
Ano: 2019 |
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Symanczik, Sarah; Courty, Pierre-Emmanuel; Boller, Thomas; Wiemken, Andreas; Al-Yahya'ei, Mohamed N.. |
Field studies have revealed the impact of changing water regimes on the structure of arbuscular mycorrhizal fungal (AMF) communities, but it is not known what happens to the abundance of individual AMF species within the community when the water conditions in the rhizosphere change. The behavior of four AMF species isolated from the Arabian desert (Diversispora aurantia, Diversispora omaniana, Septoglomus africanum, and an undescribed Paraglomus species) was investigated when assembled in microcosms containing Sorghum bicolor as host plant, and treated with various water regimes. Furthermore, the impact of invasion of these assemblages by Rhizophagus irregularis, an AMF species widely used in commercial inocula, was studied. The abundance of each AMF... |
Tipo: Journal paper |
Palavras-chave: Soil Crop health; Quality; Protection. |
Ano: 2015 |
URL: http://orgprints.org/33352/1/Symanczik-etal-2015_Mycorrhiza_Vol25_Issue8_pp639-647.pdf |
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