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The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs BJM
Coutinho,Selene Dall'Acqua; Burke,Julieta Catarina; Paula,Catia Dejuste de; Rodrigues,Miguel Trefaut; Catão-Dias,José Luiz.
Many microorganisms are able to cause diseases in amphibians, and in the past few years one of the most reported has been Batrachochytrium dendrobatidis. This fungus was first reported in Brazil in 2005; following this, other reports were made in specimens deposited in museum collections, captive and free-living frogs. The aim of this study was to compare singleplex and nested-PCR techniques to detect B. dendrobatidis in free-living and apparently healthy adult frogs from the Brazilian Atlantic Forest. The sample collection area was a protected government park, with no general entrance permitted and no management of the animals there. Swabs were taken from the skin of 107 animals without macroscopic lesions and they were maintained in ethanol p.a. Fungal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chytridiomycosis; Batrachochytrium dendrobatidis; Frogs; Brazilian Atlantic Forest; PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200551
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Susceptibility variation of Malassezia pachydermatis to antifungal agents according to isolate source BJM
Weiler,Caroline Borges; Jesus,Francielli Pantella Kunz de; Nardi,Graziela Habib; Loreto,Érico Silva; Santurio,Janio Morais; Coutinho,Selene Dall'Acqua; Alves,Sydney Hartz.
Malassezia pachydermatisis associated with dermatomycoses and otomycosis in dogs and cats. This study compared the susceptibility of M. pachydermatis isolates from sick (G1) and healthy (G2) animals to azole and polyene antifungals using the M27-A3 protocol. Isolates from G1 animals were less sensitive to amphotericin B, nystatin, fluconazole, clotrimazole and miconazole.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malassezia pachydermatis; Susceptibility; Antifungal agents.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100026
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