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Couturier, Lydie; Michel, Loic; Amaro, Teresa; Budge, Suzanne M; Da Costa, Elisabete; De Troch, Marleen; Di Dato, Valeria; Fink, Patrick; Giraldo, Carolina; Le Grand, Fabienne; Loaiza, Iván; Mathieu-resuge, Margaux; Nichols, Peter D; Parrish, Christopher C; Sardenne, Fany; Vagner, Marie; Pernet, Fabrice; Soudant, Philippe; Browman, Howard. |
Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA... |
Tipo: Text |
Palavras-chave: Aquatic nutrition; Biomarkers; Fatty acids; Marine and freshwater lipids; Methods; Recommended practices. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00651/76279/77252.pdf |
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Sardenne, Fany; Diaha, N'Guessan Constance; Amande, Monin Justin; Zudaire, Iker; Couturier, Lydie; Metral, Luisa; Le Grand, Fabienne; Bodin, Nathalie. |
In the Gulf of Guinea, bigeye Thunnus obesus (BET) and yellowfin tuna Thunnus albacares (YFT) are important for commercial fisheries and play a prominent ecological role as top predators. Using fatty acid profiles and carbon and nitrogen stable isotopes we examined their niche partitioning in this understudied region. Niche overlap was high (>70%), similar to percentages in other ocean basins. BET occupied a higher trophic position than YFT and fed on deeper prey (high δ15N values and high proportions of mono-unsaturated fatty acids). The trophic position of YFT decreased slightly in the last 15 years (δ15N values decrease ~0.5 ‰) suggesting a change in epipelagic communities, as observed in the eastern Pacific Ocean. Ontogenic changes were limited to... |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00436/54796/56254.pdf |
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